Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice.

Abstract

Heterogeneous nuclear ribonucleoprotein-M (hnRNP-M) is an abundant nuclear protein that binds to pre-mRNA and is a component of the spliceosome complex. A direct interaction was detected in vivo between hnRNP-M and the human spliceosome proteins cell division cycle 5-like (CDC5L) and pleiotropic regulator 1 (PLRG1) that was inhibited during the heat-shock stress response. A central region in hnRNP-M is required for interaction with CDC5L/PLRG1. hnRNP-M affects both 5' and 3' alternative splice site choices, and an hnRNP-M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate.

• Authors: David Llères, Marco Denegri, Marco Biggiogera, Paul Ajuh, Angus I Lamond
• Journal: EMBO reports (EMBO Rep) Vol. 11 Issue 6 Pg. 445-51 (Jun 2010) ISSN: 1469-3178 [Electronic] England
• PMID: 20467437 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CDC5L protein, human
• Cell Cycle Proteins
• Heterogeneous-Nuclear Ribonucleoprotein Group M
• Intracellular Signaling Peptides and Proteins
• Nuclear Proteins
• PLRG1 protein, human
• RNA Splice Sites
• RNA-Binding Proteins
• Recombinant Fusion Proteins
• enhanced green fluorescent protein
• Green Fluorescent Proteins

Topics

• Binding Sites
• Cell Cycle Proteins (chemistry, metabolism)
• Green Fluorescent Proteins
• HeLa Cells
• Heat-Shock Response
• Heterogeneous-Nuclear Ribonucleoprotein Group M (chemistry, metabolism)
• Humans
• Intracellular Signaling Peptides and Proteins (chemistry, metabolism)
• Nuclear Proteins (chemistry, metabolism)
• Protein Binding
• Protein Interaction Mapping
• Protein Structure, Tertiary
• RNA Splice Sites
• RNA-Binding Proteins (chemistry, metabolism)
• Recombinant Fusion Proteins