Hydroquinone, a benzene metabolite, induces Hog1-dependent stress response signaling and causes aneuploidy in Saccharomyces cerevisiae.

Abstract

Previously, we have shown that phenyl hydroquinone, a hepatic metabolite of the Ames test-negative carcinogen o-phenylphenol, efficiently induced aneuploidy in Saccharomyces cerevisiae by arresting the cell cycle at the G2/M transition as a result of the activation of the Hog1 (p38 MAPK homolog)-Swe1 (Wee1 homolog) pathway. In this experiment, we examined the aneuploidy forming effects of hydroquinone, a benzene metabolite, since both phenyl hydroquinone and hydroquinone are Ames-test negative carcinogens and share similar molecular structures. As was seen in phenyl hydroquinone, hydroquinone induced aneuploidy in yeast by delaying the cell cycle at the G2/M transition. Deficiencies in SWE1 and HOG1 abolished the hydroquinone-induced delay at the G2/M transition and aneuploidy formation. Furthermore, Hog1 was phosphorylated by hydroquinone, which may stabilize Swe1. These data indicate that the hydroquinone-induced G2/M transition checkpoint, which is activated by the Hog1-Swe1 pathway, plays a role in the formation of aneuploidy.

• Authors: Takeki Shiga, Hiroyuki Suzuki, Ayumi Yamamoto, Hiroaki Yamamoto, Kazuo Yamamoto
• Journal: Journal of radiation research (J Radiat Res) Vol. 51 Issue 4 Pg. 405-15 ( 2010) ISSN: 1349-9157 [Electronic] England
• PMID: 20467201 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cell Cycle Proteins
• Hydroquinones
• Mutagens
• Saccharomyces cerevisiae Proteins
• SWE1 protein, S cerevisiae
• Checkpoint Kinase 2
• Protein-Tyrosine Kinases
• Protein Serine-Threonine Kinases
• HOG1 protein, S cerevisiae
• Mitogen-Activated Protein Kinases
• RAD53 protein, S cerevisiae
• Benzene
• hydroquinone

Topics

• Aneuploidy
• Benzene (metabolism, toxicity)
• Cell Cycle (drug effects, genetics)
• Cell Cycle Proteins (metabolism)
• Checkpoint Kinase 2
• Genes, Fungal
• Hydroquinones (metabolism, toxicity)
• Mitogen-Activated Protein Kinases (metabolism)
• Mutagens (metabolism, toxicity)
• Protein Serine-Threonine Kinases (metabolism)
• Protein-Tyrosine Kinases (metabolism)
• Saccharomyces cerevisiae (cytology, drug effects, genetics, metabolism)
• Saccharomyces cerevisiae Proteins (metabolism)
• Signal Transduction (drug effects)
• Stress, Physiological