Nuclear-export-signal-dependent protein translocation of dUTPase encoded by Singapore grouper iridovirus.

Abstract

The dUTPase is a ubiquitous and crucial enzyme responsible for regulating cellular levels of dUTP. In the present study, the expression pattern and translocation of a dUTPase homolog encoded by Singapore grouper iridovirus (SGIV) were elucidated. The SGIV ORF049R encodes a dUTPase homolog, which is a peptide of 155 amino acids that contains five conserved motifs. The temporal expression pattern during infection in vitro revealed that the SGIV dUTPase was an early transcript. A leucine-rich nuclear export signal (NES) at the C-terminus was predicted using CBS Online Servers. Subcellular location analysis showed that SGIV dUTPase is a cytoplasmic protein. Site-direct mutagenesis by overlap extension-PCR indicated that the NES is crucial for the translocation of SGIV dUTPase from the nucleus to the cytoplasm. We have discovered for the first time that the NES-dependent translocation of dUTPase is different for SGIV than for members of other species, which depend on a nuclear localization signal. These results provide new insights into the pathogenesis of fish iridoviruses.

• Authors: Jie Gong, You-Hua Huang, Xiao-Hong Huang, Rong Zhang, Qi-Wei Qin
• Journal: Archives of virology (Arch Virol) Vol. 155 Issue 7 Pg. 1069-76 (Jul 2010) ISSN: 1432-8798 [Electronic] Austria
• PMID: 20461535 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Viral Proteins
• Pyrophosphatases
• dUTP pyrophosphatase

Topics

• Amino Acid Sequence
• Animals
• Cell Line
• Cloning, Molecular
• Computational Biology
• Fishes
• Gene Expression Profiling
• Gene Expression Regulation, Viral (physiology)
• Iridovirus (genetics, metabolism)
• Molecular Sequence Data
• Pyrophosphatases (metabolism)
• Sequence Alignment
• Viral Proteins (chemistry, genetics, metabolism)