Abstract | OBJECTIVE: PATIENTS: DESIGN: Mutational analysis was performed by automated sequencing. Loss-of-heterozygosity (LOH) analysis was carried out by sequencing and microsatellite analysis. AIP expression was assessed through quantitative PCR (qPCR) and immunohistochemistry. RESULTS: The functional inactivating mutation c.241C>T (R81X), which blocks the AIP protein from interacting with phosphodiesterase 4A (PDE4A), was identified in the heterozygous state in the leukocyte DNA of both patients. Analyzing the tumoral DNA revealed that the AIP wild-type allele was lost in the daughter's somatotropinoma and the mother's adrenocortical carcinoma. Both tumors displayed low AIP protein expression levels. Low AIP gene expression was confirmed by qPCR in the adrenocortical carcinoma. No evidence of LOH was observed in the DNA sample from the mother's B-cell lymphoma, and this tumor displayed normal AIP immunostaining. CONCLUSIONS: Our study presents the first molecular analysis of non- pituitary tumors in AIP-mutated patients. The finding of AIP inactivation in the adrenocortical tumor suggests that further investigation of the potential role of this recently identified tumor suppressor gene in non- pituitary tumors, mainly in those tumors in which the cAMP and the 11q13 locus are implicated, is likely to be worthwhile.
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Authors | Rodrigo A Toledo, Berenice B Mendonca, Maria Candida B V Fragoso, Iberê C Soares, Madson Q Almeida, Michelle B Moraes, Delmar M Lourenço Jr, Venâncio A F Alves, Marcello D Bronstein, Sergio P A Toledo |
Journal | Clinics (Sao Paulo, Brazil)
(Clinics (Sao Paulo))
Vol. 65
Issue 4
Pg. 407-15
(Apr 2010)
ISSN: 1980-5322 [Electronic] United States |
PMID | 20454499
(Publication Type: Case Reports, Journal Article)
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Chemical References |
- Intracellular Signaling Peptides and Proteins
- aryl hydrocarbon receptor-interacting protein
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Topics |
- Acromegaly
(genetics)
- Adenoma
(genetics, metabolism)
- Adolescent
- Adrenocortical Carcinoma
(genetics)
- Adult
- Female
- Gene Expression
- Genes, p53
- Germ-Line Mutation
- Growth Hormone-Secreting Pituitary Adenoma
(genetics)
- Humans
- Intracellular Signaling Peptides and Proteins
(genetics)
- Loss of Heterozygosity
(genetics)
- Multiple Endocrine Neoplasia Type 1
(genetics)
- Pituitary Neoplasms
(genetics, metabolism)
- Polymerase Chain Reaction
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