Anti-inflammatory effect of crude extract and isolated compounds from Baccharis illinita DC in acute skin inflammation.

Abstract

UNLABELLED:

ETHNOPHARMACOLOGYCAL RELEVANCE: The tea from the leaves of Baccharis illinita DC (Asteraceae family) is commonly used by the population as anti-inflammatory (including topically), protective gastric and anti-infectious. However, no studies have been done with this species to confirm its topical anti-inflammatory action.

AIM:

This study evaluated he topical effects of crude extract of leaves (CE) and its active constituents in 12-O-tetradecanoylphorbol acetate (TPA)-induced ear oedema.

METHODOLOGY:

CE and compounds effects were tested in commonly used models of TPA-, arachidonic acid (AA)- and capsaicin-ear oedema. Polymorphonuclear (PMN) cell migration was evaluated by mieloperoxidase and analyzed histologically.

RESULTS:

CE (0.1-1 mg/ear) caused a dose-related inhibition of TPA-induced ear oedema and PMN influx similarly to that produced by topical application of the steroidal anti-inflammatory drug dexamethasone. The active constituents of the AcOEt fraction kaurenoic acid, alpha-spinasterol, oleanolic acid and baurenol also inhibited TPA-induced ear edema. Histological analysis of the ear of CE-treated animals confirmed the reduction of edema and of PMN infiltration. Both CE and the nosteroidal anti-inflammatory drug indomethacin inhibited the AA-induced ear oedema, but did not change capsaicin-induced oedema.

CONCLUSION:

These results indicate that the CE and the active constituents have a topical anti-inflammatory effect and the possible mechanisms for the pharmacological effects are discussed.

• Authors: Shirley Boller, Cristian Soldi, Maria C A Marques, Elide P Santos, Daniela A Cabrini, Moacir G Pizzolatti, Aleksander R Zampronio, Michel F Otuki
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 130 Issue 2 Pg. 262-6 (Jul 20 2010) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 20452414 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2010 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Diterpenes
• Plant Preparations
• spinasterol
• Arachidonic Acid
• kaurenoic acid
• Oleanolic Acid
• Stigmasterol
• Tetradecanoylphorbol Acetate
• Capsaicin

Topics

• Administration, Topical
• Animals
• Anti-Inflammatory Agents (administration & dosage, pharmacology)
• Arachidonic Acid
• Baccharis
• Capsaicin
• Dermatitis, Contact (etiology, immunology, prevention & control)
• Disease Models, Animal
• Diterpenes (isolation & purification, pharmacology)
• Dose-Response Relationship, Drug
• Ear
• Edema (chemically induced, immunology, prevention & control)
• Male
• Mice
• Neutrophil Infiltration (drug effects)
• Oleanolic Acid (isolation & purification, pharmacology)
• Plant Leaves
• Plant Preparations (administration & dosage, chemistry, pharmacology)
• Stigmasterol (analogs & derivatives, isolation & purification, pharmacology)
• Tetradecanoylphorbol Acetate