Abstract | OBJECTIVE: METHODS: We used a murine model of 8 mins of untreated KCl- induced cardiac arrest followed by resuscitation and return of spontaneous circulation to study mitochondrial functions in four groups of animals: 1) after 8 min cardiac arrest (CA8) but no resuscitation, 2) 30 min postreturn of spontaneous circulation (R30), 3) 60 min postreturn of spontaneous circulation (R60), and in 4) shams. Heart mitochondria were immediately harvested, isolated, and stored at -80 degrees C for later spectrophotometric measurements of electron transfer activities and reactive oxygen species leakage using appropriate substrates and inhibitors. Mitochondrial cytochrome c content and tyrosine nitration were analyzed by Western blot and densitometry. RESULTS: A significant reactive oxygen species leakage from complex I was evident after just 8 min of cardiac arrest (CA8 group, p < .05), which was followed by a progressive reduction in complex I electron transfer activity (CA8 > R30 > R60). In contrast, complex II and II-III activities appeared more resistant to ischemia at the time points evaluated. Early changes in a approximately 50 kDa and approximately 25 kDa protein were observed in tyrosine nitration along with a loss of cytochrome c. CONCLUSIONS:
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Authors | Fei Han, Tong Da, Natalia A Riobo, Lance B Becker |
Journal | Critical care medicine
(Crit Care Med)
Vol. 36
Issue 11 Suppl
Pg. S447-53
(Nov 2008)
ISSN: 1530-0293 [Electronic] United States |
PMID | 20449909
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Reactive Oxygen Species
- Tyrosine
- Cytochromes c
- Hydrogen Peroxide
- Succinate Cytochrome c Oxidoreductase
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Topics |
- Animals
- Cardiopulmonary Resuscitation
- Coronary Circulation
- Cytochromes c
(metabolism)
- Electrocardiography
- Electron Transport
(physiology)
- Female
- Heart Arrest
(metabolism, physiopathology)
- Hydrogen Peroxide
(metabolism)
- Mice
- Mice, Inbred C57BL
- Mitochondria
(metabolism)
- Myocardial Ischemia
(metabolism, physiopathology)
- Myocardial Reperfusion
- Myocardium
(metabolism)
- Reactive Oxygen Species
(metabolism)
- Respiration, Artificial
- Succinate Cytochrome c Oxidoreductase
(metabolism)
- Tyrosine
(metabolism)
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