Notch-1 and notch-4 receptors as prognostic markers in breast cancer.

Abstract

BACKGROUND:

Studies looking at immunohistochemical (IHC) staining of Notch receptors in breast cancer and correlation with known prognostic factors are sparse.

METHODS:

IHC staining for nuclear, cytoplasmic, and membrane Notch-1 (N1), Notch-4 (N4), and Jagged-1 (JAG1) was performed and correlated with known prognostic factors.

RESULTS:

Of 48 breast cancers, 36 (67%) were invasive, mean age was 50 years (range 43-86 years), 37 (77%) were estrogen receptor (ERα) positive, and 13 (27%) node positive. There was significantly more marked N1 membranous staining in ERα-positive tumors (P < .05). On univariate analysis, cytoplasmic N1 was significantly correlated with node status and tumor grade (P < .05); both cytoplasmic and membranous N4 significantly correlated with Ki67 (P < .05); and membranous JAG1 significantly correlated with Ki67 (P < .05). On multivariate analysis, only cytoplasmic N1 significantly correlated with node status.

CONCLUSION:

IHC of Notch markers is feasible and correlates with known prognostic factors consistent with a biological role of Notch signaling in breast cancer progression.

• Authors: Katharine Yao, Paola Rizzo, Prabha Rajan, Kathy Albain, Karen Rychlik, Sneha Shah, Lucio Miele
• Journal: International journal of surgical pathology (Int J Surg Pathol) Vol. 19 Issue 5 Pg. 607-13 (Oct 2011) ISSN: 1940-2465 [Electronic] United States
• PMID: 20444726 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Calcium-Binding Proteins
• Intercellular Signaling Peptides and Proteins
• JAG1 protein, human
• Jagged-1 Protein
• Membrane Proteins
• NOTCH1 protein, human
• NOTCH4 protein, human
• Proto-Oncogene Proteins
• Receptor, Notch1
• Receptor, Notch4
• Receptors, Notch
• Serrate-Jagged Proteins

Topics

• Adult
• Aged
• Aged, 80 and over
• Biomarkers, Tumor (analysis)
• Breast Neoplasms (metabolism, mortality, pathology)
• Calcium-Binding Proteins (analysis, biosynthesis)
• Female
• Humans
• Immunohistochemistry
• Intercellular Signaling Peptides and Proteins (analysis, biosynthesis)
• Jagged-1 Protein
• Membrane Proteins (analysis, biosynthesis)
• Middle Aged
• Neoplasm Grading
• Neoplasm Staging
• Prognosis
• Proto-Oncogene Proteins (analysis, biosynthesis)
• Receptor, Notch1 (analysis, biosynthesis)
• Receptor, Notch4
• Receptors, Notch (analysis, biosynthesis)
• Serrate-Jagged Proteins