Abstract | BACKGROUND: Studies have documented the beneficial roles of δ opioid receptor (OR) agonist for hemorrhagic shock. However, the myocardial protection roles and the mechanisms of hemodynamic stability during resuscitation of δ-OR agonist have not been explored. This study was designed to investigate myocardial protective effects and the mechanisms of high selective δ(1) and δ(2)-OR agonists during resuscitation of acute hemorrhagic shock. MATERIALS AND METHODS: Forty-eight adult male SD rats were adopted 60-min hemorrhagic shock through removing 30% (5 mL) of the total blood volume, and followed by 2-h resuscitation with shed blood and L- lactated Ringer's solution. At the end of shock and prior to resuscitation, NS, δ(1)-OR agonist TAN-67 (10 mg/kg) and antagonist BNTX (3 mg/kg), and BNTX+TAN-67, DMSO, δ(2)-OR agonist Deltorphin II (1 mg/kg) and antagonist NTB (2 mg/kg), and NTB+Deltorphin II in 0.5 mL were administrated. Left ventricular function parameters were measured during the whole experimental period. Myocardial mitochondria were isolated to determine opening of mitochondrial permeability transition pore (mPTP). Morphologic changes in myocardium and mitochondria were observed by electron microscope. RESULTS: CONCLUSIONS:
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Authors | Xiaoli Zeng, Xiaoling Zhao, Yonghui Yang, Jianke Kuai, Changjun Gao, Daihua Yu, Hui Zhao, Wei Chai, Linong Yao |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 169
Issue 2
Pg. 267-76
(Aug 2011)
ISSN: 1095-8673 [Electronic] United States |
PMID | 20444473
(Publication Type: Journal Article)
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Copyright | Copyright © 2011 Elsevier Inc. All rights reserved. |
Chemical References |
- Mitochondrial Membrane Transport Proteins
- Mitochondrial Permeability Transition Pore
- Oligopeptides
- Quinolines
- Receptors, Opioid, delta
- TAN 67
- deltorphin II, Ala(2)-
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Topics |
- Animals
- Blood Pressure
(drug effects)
- Heart Injuries
(etiology, metabolism, prevention & control)
- Heart Rate
(drug effects)
- Male
- Mitochondrial Membrane Transport Proteins
(metabolism)
- Mitochondrial Permeability Transition Pore
- Models, Animal
- Oligopeptides
(pharmacology, therapeutic use)
- Quinolines
(pharmacology, therapeutic use)
- Rats
- Rats, Sprague-Dawley
- Receptors, Opioid, delta
(agonists)
- Resuscitation
- Shock, Hemorrhagic
(complications)
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