Abstract |
Classical swine fever (CSF) - caused by the classical swine fever virus (CSFV) - is a fatal disease of pigs that is responsible for extensive losses to the swine industry worldwide. We had demonstrated previously that a prime-boost vaccination strategy using an alphavirus (Semliki Forest virus, SFV) replicon-vectored DNA vaccine (pSFV1CS-E2) and a recombinant adenovirus (rAdV-E2) expressing the E2 glycoprotein of CSFV induced enhanced immune responses in a mouse model. In this study, we evaluated further the efficacy of the heterologous prime-boost immunization approach in pigs, the natural host of CSFV. The results showed that the pigs (n=5) receiving pSFV1CS-E2/rAdV-E2 heterologous prime-boost immunization developed significantly higher titers of CSFV-specific neutralizing antibodies and comparable CD4(+) and CD8(+) T-cell proliferation, compared to the pigs receiving double immunizations with rAdV-E2 alone. When challenged with virulent CSFV Shimen strain, the pigs of the heterologous prime-boost group did not show clinical symptoms or viremia, which were observed in one of the 5 pigs immunized with rAdV-E2 alone and all the 5 control pigs immunized with an empty adenovirus. The results demonstrate that the heterologous DNA prime and recombinant adenovirus boost strategy can induce solid protective immunity.
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Authors | Yuan Sun, Na Li, Hong-Yu Li, Miao Li, Hua-Ji Qiu |
Journal | Veterinary immunology and immunopathology
(Vet Immunol Immunopathol)
Vol. 137
Issue 1-2
Pg. 20-7
(Sep 15 2010)
ISSN: 1873-2534 [Electronic] Netherlands |
PMID | 20435352
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2010 Elsevier B.V. All rights reserved. |
Chemical References |
- Antibodies, Viral
- Vaccines, DNA
- Viral Vaccines
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Topics |
- Adenoviridae
(genetics)
- Alphavirus
(genetics)
- Animals
- Antibodies, Viral
(blood)
- Classical Swine Fever
(immunology)
- Classical Swine Fever Virus
(immunology)
- Genetic Vectors
- Immunization
- Immunization, Secondary
- Lymphocyte Activation
- Recombination, Genetic
- Replicon
- Swine
- Vaccines, DNA
(immunology)
- Viral Load
- Viral Vaccines
(immunology)
- Viremia
(immunology)
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