Abstract | OBJECTIVES: METHODS: To this end, the effect of aminooxyacetic acid hemihydrochloride (AOAA, 20 mg/kg; by intraperitoneal route, i.p.), a GABA-T inhibitor; DL-2,4-diamino-n-butyric acid hydrochloride ( DABA, 16 mg/kg; i.p.), an inhibitor of GABA uptake; and γ- aminobutyric acid ( GABA, 10 and 40 mg/kg; i.p.), diazepam (3 mg/kg; i.p.) and phenobarbital (40 mg/kg; i.p.), GABAergic agonists as well as picrotoxin (1 mg/kg; i.p.), a GABAA receptor antagonist on (PhSe)₂ (50 and 500 mg/kg, by oral route, p.o.)-induced seizures, were studied. The [(3)H] GABA uptake levels by cortical and hippocampal slices in rat pups exposed to (PhSe)₂ were also carried out. RESULTS: Pre-treatment with GABA (40 mg/kg), diazepam, phenobarbital, AOAA and DABA abolished the appearance of seizures induced by 50 mg/kg (PhSe)₂ in rat pups. Picrotoxin increased the percentage of convulsing rat pups from 42 to 100% and reduced significantly the onset for the first convulsive episode induced by (PhSe)₂ at the dose of 50 mg/kg. Diazepam and phenobarbital prolonged significantly the latency for the onset of the first convulsive episode caused by 500 mg/kg (PhSe)₂ in rat pups. [(3)H] GABA uptake levels were stimulated in cerebral cortical and hippocampal slices of convulsing rat pups administered with both doses of (PhSe)₂. DISCUSSION: Our findings demonstrated that seizures induced by (PhSe)₂ are mediated, at least in part, by an interaction with GABAergic system.
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Authors | Marina Prigol, Ethel A Wilhelm, Cristina W Nogueira, Gilson Zeni |
Journal | Neurological research
(Neurol Res)
Vol. 32
Issue 9
Pg. 1002-8
(Nov 2010)
ISSN: 1743-1328 [Electronic] England |
PMID | 20433775
(Publication Type: Journal Article)
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Chemical References |
- Acetanilides
- Benzene Derivatives
- GABA Modulators
- Organoselenium Compounds
- Picrotoxin
- diphenyldiselenide
- DABA
- gamma-Aminobutyric Acid
- Diazepam
- Phenobarbital
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Topics |
- Acetanilides
(pharmacology)
- Animals
- Animals, Newborn
- Benzene Derivatives
- Diazepam
(pharmacology)
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Drug Interactions
- GABA Modulators
(pharmacology)
- Organoselenium Compounds
- Phenobarbital
(pharmacology)
- Picrotoxin
(pharmacology)
- Rats
- Seizures
(chemically induced, metabolism)
- gamma-Aminobutyric Acid
(metabolism)
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