Anticancer activity of phenoxazines produced by bovine erythrocytes on colon cancer cells.

Abstract

The present study investigated the anticancer activity of 2-aminophenoxazine-3-one (Phx-3) and 2-amino-4,4 alpha-dihydro-4 alpha,7-dimethyl-3H-phenoxazine-3-one (Phx-1), which were obtained by improved preparation methods using bovine erythrocyte suspension, on colon cancer cell lines COLO201, DLD1 and PMCO1 in vitro. The preparation methods for Phx-1 and Phx-3 had the advantages of extensively shortening reaction time and reducing sample volumes up to one-seventh during treatment, compared with the conventional method using bovine hemoglobin solution, resulting in extensive reduction of handling time. Phx-1 and Phx-3 thus obtained were identified as pure by the absorption spectra and NMR spectra. These phenoxazines exerted strong, dose-dependent anticancer activity against colon cancer cell lines COLO201, DLD1 and PMCO1 in vitro and induced apoptosis of these cells. The present results demonstrate that Phx-1 and Phx-3, which were prepared by extensively improved methods using bovine erythrocytes, may be useful as therapeutic drugs against colon cancer that is intractable to chemotherapy.

• Authors: Takeshi Nakachi, Takafumi Tabuchi, Akira Takasaki, Sadao Arai, Keisuke Miyazawa, Akio Tomoda
• Journal: Oncology reports (Oncol Rep) Vol. 23 Issue 6 Pg. 1517-22 (Jun 2010) ISSN: 1791-2431 [Electronic] Greece
• PMID: 20428804 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 2-amino-4,4alpha-dihydro-4alpha,7-dimethyl-3H-phenoxazine-3-one
• Antineoplastic Agents
• Oxazines
• 3-aminophenoxazone

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Cattle
• Cell Proliferation (drug effects)
• Chromatography, Thin Layer
• Colonic Neoplasms (drug therapy)
• Erythrocytes (metabolism)
• Humans
• Magnetic Resonance Spectroscopy
• Oxazines (pharmacology)
• Tumor Cells, Cultured