Altered serum glycomics in Alzheimer disease: a potential blood biomarker?

Abstract	We investigated whether blood N-glycan changes can be used as a diagnostic biomarker for Alzheimer disease (AD). We used DNA sequencer-assisted, fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) technology to assay N-glycans in sera from 79 autopsy-confirmed dementia patients and 149 healthy controls. One N-glycan (NA2F) was substantially decreased in AD patients but not in controls. Use of NA2F for discriminating AD between dementia patients and healthy controls showed a diagnostic accuracy of 85.7% +/- 2.8% with 92% specificity and 70% sensitivity. The decrease in the level of NA2F in AD patients compared to non-AD patients was more pronounced in females (p < 0.0001) than in males (p < 0.014). Use of NA2F to differentiate female AD from female non-AD patients reached a diagnostic accuracy of 90.7% +/- 4.8 %. Pearson correlation analysis showed that in female dementia patients, serum NA2F levels were significantly correlated with the cerebrospinal fluid (CSF) beta-amyloid peptide of 42 amino acids (Abeta(1-42)) and tau phosphorylated at threonine 181 (P-tau(181P)) levels, whereas in male dementia patients serum NA2F levels were significantly correlated only with CSF total tau protein (T-tau) level. Thus, we suggest that the serum N-glycan marker might be suitable for longitudinal and follow-up studies.
Authors	Cuiying Chitty Chen, Sebastiaan Engelborghs, Sylviane Dewaele, Nathalie Le Bastard, Jean-Jacques Martin, Valerie Vanhooren, Claude Libert, Peter Paul De Deyn
Journal	Rejuvenation research (Rejuvenation Res) Vol. 13 Issue 4 Pg. 439-44 (Aug 2010) ISSN: 1557-8577 [Electronic] United States
PMID	20426627 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Biomarkers Carbohydrates
Topics	Adult Alzheimer Disease (blood, cerebrospinal fluid, diagnosis) Biomarkers (analysis, blood, cerebrospinal fluid) Carbohydrates (analysis, blood, cerebrospinal fluid) Case-Control Studies Electrophoresis (methods) Enzyme-Linked Immunosorbent Assay Female Glycomics Humans Male Middle Aged Sensitivity and Specificity

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