Abstract |
The medicinal herb Jinpi, derived from the dried stem barks of Fraxinus rhynchophylla belonging to Oleaceae is widely used as a variety of Korean folk remedies for anti-inflammatory, febricide, antidiarrhea, and antileukorrhea diseases. In the course of screening antidementia agents from natural products, F. rhynchophylla showed significant inhibitory activity toward Abeta(25-35)-induced neuronal cell death. An active principle was isolated and identified as syringin. When the neuroblastoma cells were exposed to 50 microM Abeta(25-35), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction rate (survival rate) decreased to 60.21 +/- 2.16% over control while syringin treated ones recovered cell viability up to 79.12 +/- 1.39% at 20 microM. In addition, 20 microM syringin almost completely removed Abeta(25-35)-induced reactive oxygen species. The neuroprotective effect of syringin seemed to be originated from the reduction of apoptosis since decrease in caspase-3 activity and expression, reduction in cleaved PARP, and DNA fragmentation were observed. These results suggest that F. rhynchophylla and syringin are expected to be useful for preventing Abeta(25-35)-induced neuronal cell damage.
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Authors | Eun-Ju Yang, Sang-In Kim, Hyun-Yeong Ku, Dong-Seok Lee, Jong-Won Lee, Yae-Sil Kim, Yeon-Hee Seong, Kyung-Sik Song |
Journal | Archives of pharmacal research
(Arch Pharm Res)
Vol. 33
Issue 4
Pg. 531-8
(Apr 2010)
ISSN: 1976-3786 [Electronic] Korea (South) |
PMID | 20422361
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Amyloid beta-Peptides
- Free Radical Scavengers
- Glucosides
- Neuroprotective Agents
- Peptide Fragments
- Phenylpropionates
- Reactive Oxygen Species
- amyloid beta-protein (25-35)
- Caspase 3
- syringin
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Topics |
- Amyloid beta-Peptides
(toxicity)
- Animals
- Apoptosis
(drug effects)
- Blotting, Western
- Caspase 3
(metabolism)
- Cell Culture Techniques
- Cell Line, Tumor
- Cell Survival
(drug effects)
- DNA Fragmentation
(drug effects)
- Dose-Response Relationship, Drug
- Fraxinus
(chemistry)
- Free Radical Scavengers
(isolation & purification, pharmacology)
- Glucosides
(isolation & purification, pharmacology)
- Magnetic Resonance Spectroscopy
- Molecular Structure
- Neurons
(drug effects, pathology)
- Neuroprotective Agents
(isolation & purification, pharmacology)
- Peptide Fragments
(toxicity)
- Phenylpropionates
(isolation & purification, pharmacology)
- Plant Bark
(chemistry)
- Plant Stems
(chemistry)
- Rats
- Reactive Oxygen Species
(metabolism)
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