HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Syringin from stem bark of Fraxinus rhynchophylla protects Abeta(25-35)-induced toxicity in neuronal cells.

Abstract
The medicinal herb Jinpi, derived from the dried stem barks of Fraxinus rhynchophylla belonging to Oleaceae is widely used as a variety of Korean folk remedies for anti-inflammatory, febricide, antidiarrhea, and antileukorrhea diseases. In the course of screening antidementia agents from natural products, F. rhynchophylla showed significant inhibitory activity toward Abeta(25-35)-induced neuronal cell death. An active principle was isolated and identified as syringin. When the neuroblastoma cells were exposed to 50 microM Abeta(25-35), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction rate (survival rate) decreased to 60.21 +/- 2.16% over control while syringin treated ones recovered cell viability up to 79.12 +/- 1.39% at 20 microM. In addition, 20 microM syringin almost completely removed Abeta(25-35)-induced reactive oxygen species. The neuroprotective effect of syringin seemed to be originated from the reduction of apoptosis since decrease in caspase-3 activity and expression, reduction in cleaved PARP, and DNA fragmentation were observed. These results suggest that F. rhynchophylla and syringin are expected to be useful for preventing Abeta(25-35)-induced neuronal cell damage.
AuthorsEun-Ju Yang, Sang-In Kim, Hyun-Yeong Ku, Dong-Seok Lee, Jong-Won Lee, Yae-Sil Kim, Yeon-Hee Seong, Kyung-Sik Song
JournalArchives of pharmacal research (Arch Pharm Res) Vol. 33 Issue 4 Pg. 531-8 (Apr 2010) ISSN: 1976-3786 [Electronic] Korea (South)
PMID20422361 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Amyloid beta-Peptides
  • Free Radical Scavengers
  • Glucosides
  • Neuroprotective Agents
  • Peptide Fragments
  • Phenylpropionates
  • Reactive Oxygen Species
  • amyloid beta-protein (25-35)
  • Caspase 3
  • syringin
Topics
  • Amyloid beta-Peptides (toxicity)
  • Animals
  • Apoptosis (drug effects)
  • Blotting, Western
  • Caspase 3 (metabolism)
  • Cell Culture Techniques
  • Cell Line, Tumor
  • Cell Survival (drug effects)
  • DNA Fragmentation (drug effects)
  • Dose-Response Relationship, Drug
  • Fraxinus (chemistry)
  • Free Radical Scavengers (isolation & purification, pharmacology)
  • Glucosides (isolation & purification, pharmacology)
  • Magnetic Resonance Spectroscopy
  • Molecular Structure
  • Neurons (drug effects, pathology)
  • Neuroprotective Agents (isolation & purification, pharmacology)
  • Peptide Fragments (toxicity)
  • Phenylpropionates (isolation & purification, pharmacology)
  • Plant Bark (chemistry)
  • Plant Stems (chemistry)
  • Rats
  • Reactive Oxygen Species (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: