Abstract | OBJECTIVE: RESEARCH DESIGN AND METHODS: Volunteers with normal condition, pre-diabetes, and diabetes were recruited through a National Institutes of Health (National Institute of Diabetes and Digestive and Kidney Diseases) study and at the Johns Hopkins Comprehensive Diabetes Center. Erythrocyte proteins were extracted and hemoglobins were depleted. Global O-GlcNAcylation of erythrocyte proteins was confirmed by Western blotting using an O-GlcNAc-specific antibody. Relative OGT and O-GlcNAcase protein amounts were determined by Western blot analysis. Relative expression of O-GlcNAcase was compared with the level of A1C. RESULTS: Erythrocyte proteins are highly O-GlcNAcylated. O-GlcNAcase expression is significantly increased in erythrocytes from both individuals with pre-diabetes and diabetes compared with normal control subjects. Unlike O-GlcNAcase, protein levels of OGT did not show significant changes. CONCLUSIONS:
O-GlcNAcase expression is increased in erythrocytes from both individuals with pre-diabetes and individuals with less well-controlled diabetes. These findings, together with the previous study that demonstrated the increased site-specific O-GlcNAcylation of certain erythrocyte proteins, suggest that the upregulation of O-GlcNAcase might be an adaptive response to hyperglycemia-induced increases in O-GlcNAcylation, which are likely deleterious to erythrocyte functions. In any case, the early and substantial upregulation of O-GlcNAcase in individuals with pre-diabetes may eventually have diagnostic utility.
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Authors | Kyoungsook Park, Christopher D Saudek, Gerald W Hart |
Journal | Diabetes
(Diabetes)
Vol. 59
Issue 7
Pg. 1845-50
(Jul 2010)
ISSN: 1939-327X [Electronic] United States |
PMID | 20413512
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
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Topics |
- Acetylglucosaminidase
(metabolism)
- Adult
- Aged
- Blotting, Western
- Diabetes Mellitus, Type 1
(enzymology)
- Diabetes Mellitus, Type 2
(enzymology)
- Erythrocytes
(enzymology)
- Female
- Humans
- Insulin Resistance
- Male
- Middle Aged
- Pilot Projects
- Prediabetic State
(enzymology)
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