Sclareol modulates the Treg intra-tumoral infiltrated cell and inhibits tumor growth in vivo.

Abstract

A regulatory or suppressor T cell is functionally defined as a T cell that inhibits an immune response by influencing the activity of another cell type. On the other hand, Th1 cells express IFN-gamma and mediate cellular immunity. Sclareol exhibits growth inhibition and cytotoxic activity against a variety of human cancer cell lines. In the first set of experiments, Sclareol was isolated from the plant Salvia sclarea and our study assessed the immuno-therapeutic effectiveness of Sclareol by direct intra-tumoral injection. Secondly, several immunological parameters such as splenocytes proliferation, intra-tumor CD4+CD25+Foxp3+ Treg cells, IFN-gamma and IL-4 secretion and tumor size were assessed to evaluate the anti-tumoral immune response. By all means, the findings confirmed that the activity of Sclareol could reduce the tumor growth in vivo against breast cancer.

• Authors: Shokoofe Noori, Zuhair M Hassan, Mehdi Mohammadi, Zohre Habibi, Nooshin Sohrabi, Saeed Bayanolhagh
• Journal: Cellular immunology (Cell Immunol) Vol. 263 Issue 2 Pg. 148-53 ( 2010) ISSN: 1090-2163 [Electronic] Netherlands
• PMID: 20409537 (Publication Type: Journal Article)
• Copyright: Copyright (c) 2010. Published by Elsevier Inc.

Chemical References

• Antineoplastic Agents
• Cytokines
• Diterpenes
• Interleukin-4
• Interferon-gamma
• sclareol

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Breast Neoplasms
• Cell Proliferation (drug effects)
• Cells, Cultured
• Cytokines (metabolism)
• Diterpenes (pharmacology)
• Female
• Flow Cytometry
• Injections, Intralesional
• Interferon-gamma (metabolism)
• Interleukin-4 (metabolism)
• Magnetic Resonance Spectroscopy
• Mice
• Mice, Inbred BALB C
• Phytotherapy
• Sheep
• T-Lymphocytes, Regulatory (drug effects, immunology)