To investigate the effect of
Dachengqi decoction on
NF-kappaB p65 expression in lung of rats with partial
intestinal obstruction and the underlying mechanism, 30 SD rats were randomly divided into three groups:
sham-operation group, model group and
Dachengqi decoction treatment group (Dachengqi group), with 10 animals in each group. The models were made by partially ligating their large intestines outside the body. The pathological changes were analyzed by HE staining. The expression of
NF-kappaB p65 in rats lung were measured by using real-time polymerase chain reaction and immunohistochemistry respectively. Moreover, the expression of
caveolin-1 in rats lung was also measured to. Increased
edema, interstitial thickening,
hemorrhage, and infiltration of inflammatory cells were found in the model group. In contrast, this change was significantly reduced in Dachengqi group as compared with model group. In addition, the up-regulated
caveolin-1 and
NF-kappaB p65 were also suppressed by
Dachengqi decoction in lung of rats with partial
intestinal obstruction. We are led to concluded that the caveolin-1-NF-kappaB pathway plays an important role in the development of
lung injury of rats with partial
intestinal obstruction and
Dachengqi decoction could down-regulate the expression of
caveolin-1 and
NF-kappaB p65 in lung of rats with partial
intestinal obstruction.