Microbiota in pediatric inflammatory bowel disease.

Abstract

OBJECTIVE:

To test the hypothesis that compared with controls, children with inflammatory bowel disease (IBD) exhibit differences in the relationships between gut microbiota and disease activity.

STUDY DESIGN:

Children and adolescents (n = 69; median age, 14 years) with IBD and 25 healthy controls (median age, 14 years) were recruited for the study. The disease activity was determined according to the Pediatric Ulcerative Colitis Activity Index or the Pediatric Crohn Disease Activity Index. Cell counts of 9 bacterial groups and species in the fecal microbiota were monitored by real-time polymerase chain reaction analysis.

RESULTS:

Although no major changes were observed in patients with ulcerative colitis, except for a decrease in bifidobacteria in the active state of IBD, children with active and inactive Crohn's disease (CD) had lower numbers of Faecalibacterium prausnitzii and bifidobacteria (P <.05), and patients with active CD had higher numbers of Escherichia coli (P <.05).

CONCLUSIONS:

The microbiota in children with CD is characterized by decreased numbers of F praunsitzii and increased numbers of E coli.

• Authors: Andreas Schwiertz, Manuela Jacobi, Julia-Stefanie Frick, Markus Richter, Kerstin Rusch, Henrik Köhler
• Journal: The Journal of pediatrics (J Pediatr) Vol. 157 Issue 2 Pg. 240-244.e1 (Aug 2010) ISSN: 1097-6833 [Electronic] United States
• PMID: 20400104 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright (c) 2010 Mosby, Inc. All rights reserved.

Chemical References

• DNA, Bacterial

Topics

• Adolescent
• Adult
• Case-Control Studies
• Child
• Child, Preschool
• Colitis, Ulcerative (microbiology)
• Crohn Disease (microbiology)
• DNA, Bacterial (metabolism)
• Escherichia coli (metabolism)
• Humans
• In Situ Hybridization, Fluorescence
• Infant
• Inflammatory Bowel Diseases (microbiology)
• Intestines (microbiology)
• Reverse Transcriptase Polymerase Chain Reaction
• Ruminococcus (metabolism)