Antifreeze protein suppresses spontaneous neural activity and protects neurons from hypothermia/re-warming injury.

Abstract

Antifreeze proteins (AFP) are associated with protection from freezing. We measured the effect of type I antifreeze protein on spontaneous bursting of mixed neuronal/glial cultures using a multi-electrode array culture system. Antifreeze protein (10mg/ml) reversibly depressed bursting activity without inhibiting mitochondrial oxidative capacity. The effect of antifreeze protein on cold/re-warming injury was investigated in rat hippocampal slice cultures. Compared to bovine serum albumin at a similar concentration, antifreeze protein protected hippocampal neurons from 8h of profound hypothermia at (4 degrees C) followed by re-warming. The protection observed is believed to be associated with the inhibitory effect of antifreeze protein.

• Authors: Liel Rubinsky, Nadav Raichman, Jacob Lavee, Hanan Frenk, Eshel Ben-Jacob, Philip E Bickler
• Journal: Neuroscience research (Neurosci Res) Vol. 67 Issue 3 Pg. 256-9 (Jul 2010) ISSN: 1872-8111 [Electronic] Ireland
• PMID: 20398707 (Publication Type: Journal Article)
• Copyright: Copyright 2010 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

Chemical References

• Antifreeze Proteins, Type I
• Indicators and Reagents
• Neuroprotective Agents
• Tetrazolium Salts
• 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide

Topics

• Animals
• Antifreeze Proteins, Type I (pharmacology)
• Cold Temperature (adverse effects)
• Flounder (metabolism)
• Hippocampus (drug effects, physiology)
• Hypothermia, Induced (adverse effects)
• Indicators and Reagents
• Mitochondria (drug effects, metabolism)
• Nerve Net (drug effects)
• Neurons (drug effects)
• Neuroprotective Agents
• Organ Culture Techniques
• Oxidation-Reduction
• Rats
• Rats, Sprague-Dawley
• Rewarming (adverse effects)
• Tetrazolium Salts