Synthetic CDCA derivatives-induced apoptosis of stomach cancer cell line SNU-1 cells.

Abstract

PURPOSE:

This study was conducted to explore whether CDCA derivatives induce apoptosis in a stomach cancer cell line, and to dissect the detailed mechanism underlying apoptosis.

MATERIALS AND METHODS:

The human stomach cancer cell line, SNU-1, cells were treated with the synthetic CDCA derivatives, HS-1199 and HS-1200. DNA and mitochondrial stains were used to detect apoptotic cells by fluorescence imaging or flow cytometry. The caspase-3 activity was measured by Western blotting.

RESULTS:

Both the HS-1199 and HS-1200 induced decreased viabilities of the SNU-1 cells, in time-dependent manners. The CDCA derivatives demonstrated various apoptosis hallmarks, such as mitochondrial changes (reduction of MMP, cytochrome c release, and Smac/ DIABLO translocation), activation of caspase-3 (resulting in the degradation of PARP and DFF45), DNA fragmentation and nuclear condensation.

CONCLUSION:

The CDCA derivatives, HS-1199 and HS-1200, both induced apoptosis of the SNU-1 gastric cancer cells in caspase- and mitochondria-dependent fashions. Many important issues relating to their therapeutic applications remain to be elucidated.

• Authors: Bongkyung Moon, Min-Chan Kim, Joo-sung Park
• Journal: Cancer research and treatment (Cancer Res Treat) Vol. 36 Issue 2 Pg. 132-9 (Apr 2004) ISSN: 2005-9256 [Electronic] Korea (South)
• PMID: 20396553 (Publication Type: Journal Article)