Reduced CD27 expression on antigen-specific CD4+ T cells correlates with persistent active tuberculosis.

Abstract

OBJECTIVE:

CD27, a member of the tumor necrosis factor receptor family, has important role in generation of T cell immunity. In this study, association of CD27 expression on mycobacterial antigen-specific CD4+ T cells with pulmonary tuberculosis (TB) was investigated.

METHODS:

Mycobacterial antigen-specific CD4+ T cells were identified based on CD154 expression and CD27 expression on antigen-specific CD4 T cells was analyzed by flow cytometry.

RESULTS:

Compared with tuberculin-positive controls, patients with bacterial culture-positive pulmonary TB had significantly reduced CD27 expression on antigen-specific CD4 T cells. The persistent active TB patients had much lower percentages of CD27+ antigen-specific CD4 T cells than culture-positive new TB patients (P=0.008) and healthy controls (P=0.005). Logistic regression analysis on frequencies of CD27-expressing antigen-specific CD4 T cells and TB patients' clinical characteristics indicated that low percentage of CD27+ antigen-specific CD4 T cells correlated significantly with persistent active tuberculosis (P=0.002, odds ratio=19.6).

CONCLUSION:

It is concluded that frequency of CD27+ antigen-specific CD4 T cells could be used as an immunological marker for persistent active TB.

• Authors: Jing Jiang, Xianyuan Wang, Xinjing Wang, Zhihong Cao, Yanhua Liu, Mei Dong, Aihua Tong, Xiaoxing Cheng
• Journal: Journal of clinical immunology (J Clin Immunol) Vol. 30 Issue 4 Pg. 566-73 (Jul 2010) ISSN: 1573-2592 [Electronic] Netherlands
• PMID: 20393787 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Tuberculin
• Tumor Necrosis Factor Receptor Superfamily, Member 7

Topics

• Adult
• Biomarkers (analysis)
• CD4-Positive T-Lymphocytes (immunology)
• Case-Control Studies
• Female
• Humans
• Male
• Middle Aged
• Mycobacterium (immunology)
• T-Cell Antigen Receptor Specificity (immunology)
• Tuberculin (analysis)
• Tuberculosis (diagnosis, immunology)
• Tumor Necrosis Factor Receptor Superfamily, Member 7 (analysis, deficiency)
• Young Adult