Rapid diagnosis of Old World Leishmaniasis by high-resolution melting analysis of the 7SL RNA gene.

Abstract

High-resolution melt analysis PCR (HRM PCR) for diagnosis of Old World Leishmania was developed using the 7SL RNA gene. Cutaneous leishmaniasis samples were analyzed. Sensitivity and specificity of HRM PCR were significantly better (P < 0.001) than those of internal transcribed spacer 1 PCR and similar to those of kinetoplast DNA PCR.

• Authors: Abedelmajeed Nasereddin, Charles L Jaffe
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 48 Issue 6 Pg. 2240-2 (Jun 2010) ISSN: 1098-660X [Electronic] United States
• PMID: 20392923 (Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 7SL RNA
• DNA Primers
• RNA, Protozoan
• RNA, Small Cytoplasmic
• Signal Recognition Particle

Topics

• DNA Primers (genetics)
• Humans
• Leishmania (genetics, isolation & purification)
• Leishmaniasis, Cutaneous (diagnosis, parasitology)
• Parasitology (methods)
• Polymerase Chain Reaction (methods)
• RNA, Protozoan (genetics)
• RNA, Small Cytoplasmic (genetics)
• Sensitivity and Specificity
• Signal Recognition Particle (genetics)
• Transition Temperature