Control of Salmonella enteritidis infections in poultry by polymyxin B and trimethoprim.

Antimicrobial compounds were screened in vitro in Trypticase soy broth for antimicrobial activity against a virulent strain of Salmonella enteritidis. Of the several compounds tested, polymyxin B showed the strongest inhibition in vitro, preventing growth at a concentration of less than or equal to 10 micrograms/ml. Polymyxin B administered in the drinking water was effective in vivo for preventing infections in 1-day-old chickens but did not remove established infections in 1-week-old chickens. It was found that trimethoprim, which was not active in vitro, prevented colonization and removed existing infections in 1-day-old chickens when it was administered together with polymyxin B sulfate. Enrichment cultures in which selenite-cystine and tetrathionate broth media were used showed that chickens given a combination of 100 micrograms of polymyxin B sulfate per ml and 250 micrograms of trimethoprim per ml 24 h prior to oral inoculation with 10(8) to 10(9) CFU were negative for S. enteritidis after 7 days. Established infections (10(5) to 10(6) CFU/g of feces) in 1-week-old chickens were eliminated by treatment with the polymyxin-trimethoprim system. This antimicrobial agent treatment may be useful for preventing colonization in poultry and for eliminating S. enteritidis from infected flocks.
AuthorsM C Goodnough, E A Johnson
JournalApplied and environmental microbiology (Appl Environ Microbiol) Vol. 57 Issue 3 Pg. 785-8 (Mar 1991) ISSN: 0099-2240 [Print] UNITED STATES
PMID2039233 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Polymyxin B
  • Trimethoprim
  • Animals
  • Chickens
  • Drug Resistance, Microbial
  • Drug Therapy, Combination (administration & dosage)
  • Polymyxin B (administration & dosage)
  • Poultry Diseases (drug therapy, prevention & control)
  • Salmonella Infections, Animal (drug therapy, prevention & control)
  • Salmonella enteritidis (drug effects)
  • Trimethoprim (administration & dosage)

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