Development and evaluation of porcine cysticercosis QuickELISA in Triturus EIA analyzer.

Abstract

We evaluated three diagnostic antigens (recombinant GP50, recombinant T24H, and synthetic Ts18var1) for cysticercosis and found that all three performed well in detecting cysticercosis in humans and pigs in several assay formats. These antigens were adapted to a new antibody detection format (QuickELISA). With one single incubation step which involves all reactants except the enzyme substrate, the QuickELISA is particularly suited for automation. We formatted the QuickELISA for the Triturus EIA analyzer for testing large numbers of samples. We found that in QuickELISA formats rGP50 and rT24H have better sensitivity and specificity than sTs18var1 for detecting porcine cysticercosis.

• Authors: Sukwan Handali, Sowmya Pattabhi, Yeuk-Mui Lee, Maria Silva-Ibanez, Victor A Kovalenko, Andrew E Levin, Armando E Gonzalez, Jacquelin M Roberts, Hector H Garcia, Robert H Gilman, Kathy Hancock, Victor C W Tsang
• Journal: Journal of immunoassay & immunochemistry (J Immunoassay Immunochem) Vol. 31 Issue 1 Pg. 60-70 ( 2010) ISSN: 1532-4230 [Electronic] England
• PMID: 20391018 (Publication Type: Journal Article)

Topics

• Animals
• Cysticercosis (diagnosis, veterinary)
• Enzyme-Linked Immunosorbent Assay (instrumentation, methods)
• Sensitivity and Specificity
• Swine
• Swine Diseases (diagnosis)