The study was designed to investigate the role of three
proteolytic enzymes viz.,
chymotrypsin, trypsin and
serratiopeptidase on hind paw
edema and cotton pellet induced
granuloma and their possible interactions with
aspirin in albino rats. Animals were treated with
proteolytic enzymes alone in three different doses or
aspirin or in combination with subantiinflammatory dose of
aspirin or saline, 30 min before injection of 0.1ml 1%
carrageenan. Paw volume was measured before and 3 h after the injection of
carrageenan.
Chymotrypsin, (5, 18 and 36 mg/kg),
trypsin (1.44, 2.88 and 5.76 mg/kg) and
serratiopeptidase (0.45, 0.9 and 2.70 mg/kg) were showed dose dependent antiinflammatory activity in acute model of
inflammation.
Serratiopeptidase showed better antiinflammatory activity on
carrageenan induced
inflammation than other two
proteolytic enzymes and
aspirin. However,
chymotrypsin and
serratiopeptidase were found to be more effective than
aspirin in subacute model of
inflammation.
Chymotrypsin, trypsin and
serratiopeptidase possess antiinflammatory activity and exhibit synergistic effect with
aspirin in both acute and subacute models of
inflammation in rats.