Abstract |
Effects of heptachlor, an organochlorine pesticide, on human myeloblastic leukemia ML-1 cells were determined. Similar to 12-O-tetradecanoylphorbol-13-acetate (TPA), a known tumor promoter, heptachlor induced cell adherence and formation of extended cytoplasmic pseudopodia in ML-1 cells. The growth of ML-1 was slightly stimulated by low concentrations (less than 30 nM) of heptachlor. A dose-responsive cell death was also observed when ML-1 cells were treated with heptachlor at concentrations greater than 80 microM. Examination by light microscopy of the cells treated with 80 microM heptachlor revealed a gradual appearance of differentiation characteristics in the culture. On Day 3 of the treatment, 41% of the cells remained unchanged as ML-1, 39% of the cells showed changes and apparent cell differentiation, and 20% of the cells were induced to differentiate to monocyte- or macrophage-like cell type. Electron microscopy also revealed cellular differentiation and the presence of monocyte- and macrophage-like cell types (22%) was confirmed by positive esterase staining.
|
Authors | L F Chuang, D E Hinton, A T Cheung, R Y Chuang |
Journal | Toxicology and applied pharmacology
(Toxicol Appl Pharmacol)
Vol. 109
Issue 1
Pg. 98-107
(Jun 01 1991)
ISSN: 0041-008X [Print] United States |
PMID | 2038753
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
|
Chemical References |
- Carcinogens
- Heptachlor
- Naphthol AS D Esterase
|
Topics |
- Acute Disease
- Carcinogens
- Cell Differentiation
(drug effects)
- Cell Division
(drug effects)
- Cell Survival
(drug effects)
- Heptachlor
(pharmacology)
- Humans
- Leukemia, Myeloid
(enzymology, pathology)
- Microscopy
- Naphthol AS D Esterase
(metabolism)
- Tumor Cells, Cultured
|