HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

IgA detection in human neurocysticercosis using different preparations of heterologous antigen.

Abstract
Neurocysticercosis (NC) is the most important neurological disease of parasitic origin in humans. IgA and IgG detection in serum from neurocysticercosis patients was tested using some antigenic preparations of total saline extract from Taenia saginata: detergent (D) and aqueous (A) phases extracted with Triton X-114 and the jacalin bound (JBF) and unbound fractions (JUF) obtained by affinity chromatography using jacalin column. Samples were obtained from 45 patients with definitive NC, who were subdivided into active-NC group and inactive-NC group; 35 patients with other parasitoses; and 30 apparently healthy individuals. Sensitivity and specificity were calculated. Specificity to detect IgA and IgG for D phase, respectively, were 89.8% and 86.9% and for IgG detection 91.3% and 76.8% when using D phase and JUF, respectively. D phase and JBF proved to be specific and efficient and could be efficiently utilized as an alternative antigen for IgA detection in NC, with comparable results with IgG.
AuthorsVanessa da S Ribeiro, Marianna N Manhani, Julia M Costa-Cruz
JournalParasitology research (Parasitol Res) Vol. 107 Issue 1 Pg. 221-5 (Jun 2010) ISSN: 1432-1955 [Electronic] Germany
PMID20386922 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Helminth
  • Antigens, Helminth
  • Antigens, Heterophile
  • Immunoglobulin A
  • Immunoglobulin G
Topics
  • Animals
  • Antibodies, Helminth (blood)
  • Antigens, Helminth (isolation & purification)
  • Antigens, Heterophile (isolation & purification)
  • Cell Fractionation
  • Chromatography, Affinity
  • Humans
  • Immunoglobulin A (blood)
  • Immunoglobulin G (blood)
  • Neurocysticercosis (diagnosis, immunology)
  • Parasitology (methods)
  • Sensitivity and Specificity
  • Taenia saginata (chemistry, immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: