Abstract | BACKGROUND: METHODS: A bladder cell line was engineered to express a doxycycline (dox) regulated shRNA against PSCA. To shed light on the PSCA biological role in tumor growth, microarray analysis was carried out as a function of PSCA expression. Expression of gene set of interest was further analyzed by qPCR RESULTS: Down regulation of the PSCA expression was associated with reduced cell proliferation in vitro and in vivo. Mice bearing subcutaneous tumors showed a reduced tumor growth upon treatment with dox, which effectively induced shRNA against PSCA as revealed by GFP expression. Pathway analysis of deregulated genes suggests a statistical significant association between PSCA downregulation and activation of genes downstream of the IFNalpha/beta receptor. CONCLUSIONS: These experiments established for the first time a correlation between the level of PSCA expression and tumor growth and suggest a role of PSCA in counteracting the natural immune response.
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Authors | Emanuele Marra, Paolo Uva, Valentina Viti, Valeria Simonelli, Eugenia Dogliotti, Emanuele De Rinaldis, Armin Lahm, Nicola La Monica, Alfredo Nicosia, Gennaro Ciliberto, Fabio Palombo |
Journal | BMC cancer
(BMC Cancer)
Vol. 10
Pg. 129
(Apr 07 2010)
ISSN: 1471-2407 [Electronic] England |
PMID | 20374648
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Neoplasm
- GPI-Linked Proteins
- Membrane Glycoproteins
- Neoplasm Proteins
- PSCA protein, human
- Doxycycline
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Topics |
- Animals
- Antigens, Neoplasm
- Cell Growth Processes
(physiology)
- Cell Line, Tumor
- Clone Cells
- Down-Regulation
- Doxycycline
(pharmacology)
- Female
- GPI-Linked Proteins
- Humans
- Membrane Glycoproteins
(biosynthesis, genetics, immunology)
- Mice
- Mice, Nude
- Neoplasm Proteins
(biosynthesis, genetics, immunology)
- Polymerase Chain Reaction
- RNA Interference
- Signal Transduction
(immunology)
- Transcription, Genetic
- Urinary Bladder Neoplasms
(genetics, immunology, metabolism, pathology)
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