[Toxic effect of immunosuppression agent Everolimus on insuloma cells and pancreatic islets of SD rat].

Abstract

OBJECTIVE:

To investigate the effect of immunosuppression agent Everolimus on the viability and function of insuloma cells (INS-1) and pancreatic islet cells cultured in vitro.

METHODS:

INS-1 cells and islets were treated with a series of concentrations of immunosuppression agents (Everolimus, Cyclosporin A, Sirolimus and Mycophenolate Mofetil). The viability of INS-1 cells and rat pancreatic islets were determined with MTT and the function of INS-1 cells and rat pancreatic islets was evaluated with Glucose-stimulated insulin secretion assay.

RESULTS:

Above the clinical blood concentration, the inhibition rate of islet cell proliferation in the high concentration group of Everolimus and Sirolimus was significantly lower than that of Cyclosporin A and Mycophenolate Mofetil group (P < 0.05); Everolimus in the blood drug level, like other immunosuppressive agents, can inhibit the function of insulin secretion, and the stimulation index of each group was no significant difference.

CONCLUSION:

Compared to Mycophenolate Mofetil and Cyclosporin A, Everilimus and Sirolimus demonstrate lower toxicity effect on INS-1 cells and rat pancreatic islets in vitro and Everolimus is expected as a new type of immunosuppressive agent used in clinical islet transplantation.

• Authors: Xi Jin, Cong Cong, Yu Yuan, Gang Mai, Ling-Ling Wei, You-Nan Chen, Jing-Qiu Cheng, Yan-Rong Lu
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 41 Issue 1 Pg. 68-72 (Jan 2010) ISSN: 1672-173X [Print] China
• PMID: 20369473 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Immunosuppressive Agents
• Insulin
• Cyclosporine
• Everolimus
• Mycophenolic Acid
• Sirolimus

Topics

• Animals
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival
• Cells, Cultured
• Cyclosporine (adverse effects, pharmacology)
• Everolimus
• Immunosuppressive Agents (adverse effects, pharmacology)
• Insulin (metabolism)
• Insulin Secretion
• Insulinoma (pathology)
• Islets of Langerhans (cytology, drug effects, physiology)
• Mycophenolic Acid (adverse effects, analogs & derivatives, pharmacology)
• Pancreatic Neoplasms (pathology)
• Rats
• Sirolimus (adverse effects, analogs & derivatives, pharmacology)