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Danthron induces DNA damage and inhibits DNA repair gene expressions in GBM 8401 human brain glioblastoma multiforms cells.

Abstract
Our primary studies had shown that danthron induced cytotoxic effects, including apoptosis and inhibition of migration and invasion. However, danthron-affected DNA damage and repair gene expressions are not clear. In this study, we investigated to examine whether or not danthron induced DNA damage and inhibited DNA repair gene expression in human brain glioblastoma multiforms (GBM 8401) cells. The results from Comet assay indicated that incubation of GBM 8401 cells with 0, 50, 100 and 150 microM of danthron led to a longer DNA migration smear based on the single cell electrophoresis (Comet tail). The results from real-time PCR assay demonstrated that 100 microM of danthron for 24 h treatment in GBM 8401 cells led to decrease all examined ataxia telangiectasia mutated (ATM), ataxia-telangiectasia and Rad3-related (ATR), breast cancer 1, early onset (BRCA-1), 14-3-3 proteins sigma (14-3-3sigma), DNA-dependent serine/threonine protein kinase (DNA-PK) and O ( 6 )-methylguanine-DNA methyltransferase (MGMT) mRNA expressions. Taken together, the present study showed that danthron caused DNA damage and inhibited DNA repair genes, which may be the factors for danthron-inhibited cell growth in vitro.
AuthorsHsu-Feng Lu, Tung-Yuan Lai, Te-Chung Hsia, Yih-Jing Tang, Jai-Sing Yang, Jo-Hua Chiang, Chi-Cheng Lu, Chi-Ming Liu, Hai-Lung Wang, Jing-Gung Chung
JournalNeurochemical research (Neurochem Res) Vol. 35 Issue 7 Pg. 1105-10 (Jul 2010) ISSN: 1573-6903 [Electronic] United States
PMID20369292 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anthraquinones
  • Antineoplastic Agents, Phytogenic
  • danthron
Topics
  • Anthraquinones (adverse effects)
  • Antineoplastic Agents, Phytogenic (adverse effects)
  • Brain Neoplasms (metabolism)
  • Cell Line, Tumor
  • Cell Survival (drug effects)
  • DNA Damage (drug effects, genetics)
  • DNA Repair (drug effects, genetics)
  • Glioblastoma (metabolism)
  • Humans

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