Protective effect of gastrin-releasing peptide receptor antagonist in carrageenan-induced pleural inflammation in rats.

Abstract

OBJECTIVE:

We report the effects of the gastrin-releasing peptide (GRP) receptor antagonist RC-3095 in an acute inflammation model induced by carrageenan.

METHODS:

Male Wistar rats received saline or saline containing 2% lambda-carrageenan into the pleural cavity, with some also receiving RC-3095 3 mg/kg subcutaneously, immediately after surgery. Four hours later, the rats were killed and pleural exudate was obtained for evaluation of total cell count, lactate dehydrogenase activity, total protein, cytokines analysis and nitrite/nitrate concentrations; myeloperoxidase (MPO) activity and oxidative stress were evaluated in the lung.

RESULTS:

RC-3095 exhibited pronounced anti-inflammatory actions by inhibition of leukocyte influx and blockade of MPO, nitrite/nitrate and cytokine levels. Moreover, the results showed that RC-3095 elicits action against oxidative damage in lipids and proteins, as well as increasing cell viability.

CONCLUSION:

The present findings suggest that GRP plays a role in acute inflammation that can be related with the reduction of oxidative damage and that it could be effective in therapeutic applications.

• Authors: Fabricia Petronilho, Bruna de Souza, Francieli Vuolo, César A F Benetton, Emilio L Streck, Rafael Roesler, Gilberto Schwartsmann, Felipe Dal-Pizzol
• Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.] (Inflamm Res) Vol. 59 Issue 9 Pg. 783-9 (Sep 2010) ISSN: 1420-908X [Electronic] Switzerland
• PMID: 20364359 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Nitrates
• Nitrites
• Peptide Fragments
• Receptors, Bombesin
• bombesin (6-14), Tpi(6)-Leu(13)-psi(CH2NH)-Leu(14)-
• Carrageenan
• L-Lactate Dehydrogenase
• Peroxidase
• Bombesin

Topics

• Acute Disease
• Animals
• Anti-Inflammatory Agents (therapeutic use)
• Bombesin (analogs & derivatives, therapeutic use)
• Carrageenan (toxicity)
• Cell Movement (drug effects)
• Cytokines (analysis)
• L-Lactate Dehydrogenase (analysis)
• Leukocytes (drug effects)
• Male
• Nitrates (analysis)
• Nitrites (analysis)
• Oxidative Stress (drug effects)
• Peptide Fragments (therapeutic use)
• Peroxidase (analysis)
• Pleurisy (chemically induced, prevention & control)
• Rats
• Rats, Wistar
• Receptors, Bombesin (antagonists & inhibitors)