Abstract |
In a series of studies in SPF and conventional guinea pigs, various adjuvants ( larifan, polyoxidonium-PO, natrium thiosulphate-NT, TNF-β and Ribi adjuvant system-RAS) were evaluated for their ability to enhance immune responses to the live brucellosis vaccine, Brucella abortus strain 82-PS ( penicillin-sensitive). Combining adjuvants with S82-PS increased synthesis of antibodies against rough (R) and smooth (S) Brucella antigens. Dynamics and levels of antibodies differed dependent upon the adjuvant. Adjuvants enhanced cell-mediated responses to S82-PS, and phagocytosis by macrophages. Humoral and cellular immune responses stimulated by the adjuvants correlated with increased vaccine protection against experimental challenge. The highest protection was demonstrated by combining TNF-β or PO with S82-PS. Our data demonstrates the potential of adjuvants to improve immunogenic properties of live brucellosis vaccines.
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Authors | Alexander A Denisov, Yulia S Korobovtseva, Olga M Karpova, Alla V Tretyakova, Larisa V Mikhina, Arkadyi V Ivanov, Konstantin M Salmakov, Roman V Borovick |
Journal | Vaccine
(Vaccine)
Vol. 28 Suppl 5
Pg. F17-22
(Oct 01 2010)
ISSN: 1873-2518 [Electronic] Netherlands |
PMID | 20362624
(Publication Type: Comparative Study, Journal Article)
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Copyright | Copyright © 2010 Elsevier Ltd. All rights reserved. |
Chemical References |
- Adjuvants, Immunologic
- Antibodies, Bacterial
- Brucella Vaccine
- Cell Wall Skeleton
- Cord Factors
- Lipid A
- Lymphotoxin-alpha
- Organic Chemicals
- Piperazines
- Polymers
- Ribi adjuvant
- larifan
- azoximer bromide
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Topics |
- Adjuvants, Immunologic
(pharmacology)
- Animals
- Antibodies, Bacterial
(immunology)
- Brucella Vaccine
(immunology)
- Brucella abortus
(immunology)
- Brucellosis
(immunology, prevention & control)
- Cell Proliferation
- Cell Wall Skeleton
(immunology)
- Cord Factors
(immunology)
- Guinea Pigs
- Immunity, Cellular
- Immunity, Humoral
- Lipid A
(analogs & derivatives, immunology)
- Lymphotoxin-alpha
(immunology)
- Macrophages, Peritoneal
(immunology)
- Male
- Organic Chemicals
(immunology)
- Phagocytosis
- Piperazines
(immunology)
- Polymers
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