Neutralization of microcystin shock in mice by tumor necrosis factor alpha antiserum.

Abstract

Microcystis aeruginosa is a common cyanobacterium in water blooms that appear widely in nutrient-rich, fresh, and brackish waters, and its toxic blooms cause the death of domestic animals. The administration of a crude toxic cell extract of M. aeruginosa K-139 to mice can produce tumor necrosis factor (TNF) and prompt severe physiological disturbances, especially liver damage, which can lead to death. The in vitro production of TNF-alpha by peritoneal macrophages was observed after stimulation with the cell extract or the purified toxin from K-139 cells. The expression of a TNF-alpha mRNA was also detected in spleen cells and peritoneal macrophages after stimulation with the cell extract. However, a previous injection of rabbit anti-murine TNF-alpha serum could prevent the liver damage to some extent and protect the mice from death. These findings indicate the involvement of TNF in microcystin shock.

• Authors: Y Nakano, M Shirai, N Mori, M Nakano
• Journal: Applied and environmental microbiology (Appl Environ Microbiol) Vol. 57 Issue 1 Pg. 327-30 (Jan 1991) ISSN: 0099-2240 [Print] United States
• PMID: 2036020 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies
• Marine Toxins
• Microcystins
• Peptides, Cyclic
• Tumor Necrosis Factor-alpha
• cyanoginosin LR

Topics

• Animals
• Antibodies (administration & dosage)
• Female
• In Vitro Techniques
• Macrophages (metabolism)
• Marine Toxins (toxicity)
• Mice
• Mice, Inbred BALB C
• Microcystins
• Peptides, Cyclic (toxicity)
• Shock, Septic (etiology, physiopathology, prevention & control)
• Tumor Necrosis Factor-alpha (antagonists & inhibitors, physiology)