Abstract |
IgE-mediated food allergies are an important cause of life-threatening hypersensitivity reactions. Orally administered peanut antigens mixed with the mucosal adjuvant cholera toxin (CT) induce a strong peanut extract (PE)-specific serum IgE response that is correlated with T-helper type 1 (Th1) and type 2 (Th2)-like T-cell responses. This study was conducted to determine if respiratory enteric orphan virus (reovirus), a non-pathogenic virus that induces robust Th1-mediated mucosal and systemic responses could modulate induction of PE-specific allergic responses when co-administered with PE. Young mice were orally exposed to PE mixed with CT, reovirus, or both CT and reovirus. As expected, CT promoted PE-specific serum IgE, IgG1, and IgG2a and intestinal IgA production as well as splenic Th1- and Th2-associated cytokine recall responses. Reovirus did not alter PE-specific serum IgE and IgG1 levels, but substantially increased the PE-specific IgG2a response when co-administered with PE with or without CT. Additionally, reovirus significantly decreased the percentage of the Peyer's patch CD8+ T-cells and Foxp3+CD4+ T-regulatory cells when co-administered with PE. These results demonstrate that an acute mucosal reovirus infection and subsequent Th1 immune response is capable of modulating the Th1/Th2 controlled humoral response to PE. The reovirus-mediated increase in the PE-specific IgG2a antibody response may have therapeutic implications as increased levels of non-allergenic PE-specific IgG2a could block PE antigens from binding to IgE-sensitized mast cells.
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Authors | Ronald J Fecek, Marisa Marcondes Rezende, Ryan Busch, Ine Hassing, Raymond Pieters, Christopher F Cuff |
Journal | Immunobiology
(Immunobiology)
Vol. 215
Issue 12
Pg. 941-8
(Dec 2010)
ISSN: 1878-3279 [Electronic] Netherlands |
PMID | 20356650
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 Elsevier GmbH. All rights reserved. |
Chemical References |
- Cytokines
- Immunoglobulin A
- Immunoglobulin G
- Plant Extracts
- Cholera Toxin
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Topics |
- Animals
- Arachis
(chemistry, immunology)
- CD8-Positive T-Lymphocytes
(drug effects, metabolism, microbiology)
- Cell Line
- Cells, Cultured
- Cholera Toxin
(immunology, pharmacology)
- Cytokines
(immunology, metabolism)
- Disease Models, Animal
- Female
- Flow Cytometry
- Humans
- Immunoglobulin A
(immunology)
- Immunoglobulin G
(immunology)
- Intestinal Mucosa
(drug effects, immunology, virology)
- Intestine, Small
(drug effects, immunology, virology)
- Mice
- Mice, Inbred C3H
- Peanut Hypersensitivity
(immunology)
- Peyer's Patches
(immunology, virology)
- Plant Extracts
(immunology, pharmacology)
- Reoviridae Infections
(immunology)
- Spleen
(cytology, drug effects, metabolism)
- Th1 Cells
(drug effects, immunology)
- Tissue Culture Techniques
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