Characterisation of a haemagglutinin from Hokkaido red bean (Phaseolus vulgaris cv. Hokkaido red bean).

Abstract

BACKGROUND:

A haemagglutinin was purified from Japanese Hokkaido red beans (Phaseolus vulgaris cv. Hokkaido red bean) with a procedure that included three chromatographic media.

RESULTS:

Haemagglutinating activity was adsorbed on DEAE cellulose, Affi-gel blue gel and Mono S. The pure haemagglutinin was a homodimer and each subunit was around 30 kDa in molecular mass. The haemagglutinating activity of this agglutinin could not be inhibited by a variety of simple sugars at 200 mmol L(-1) concentration including alpha-L-fucose, D(+)-galactose, D(+)-glucose, D(+)-glucosamine, D(-)galactosamine, galacturonic acid, (+)-lactose, D(+)-melibose, L(-)-mannose, D(+)-mannose, D-mannosamine, D(+)-raffinose, L-rhamnose, (+)-xylose and galacturonic acid. The haemagglutinating activity was fully retained at pH 4-11 and at 0-80 degrees C, but was completely lost at extreme pH values (0-2 and 13-14) and at very high temperatures (90 degrees C and 100 degrees C). The haemagglutinin exhibited a weak mitogenic activity toward mouse splenocytes, a stronger anti-proliferative activity than Con A toward HepG2 (human hepatoma) cells and inhibited >80% of HIV-1 reverse transcriptase inhibitory activity at 3.3 micromol L(-1). It was devoid of anti-fungal activity.

CONCLUSION:

Hokkaido red bean haemagglutinin possesses a potent anti-proliferative effect on HepG2 cells.

• Authors: Jack H Wong, Chung T Wan, Tzi B Ng
• Journal: Journal of the science of food and agriculture (J Sci Food Agric) Vol. 90 Issue 1 Pg. 70-7 (Jan 15 2010) ISSN: 1097-0010 [Electronic] England
• PMID: 20355014 (Publication Type: Journal Article)
• Copyright: Copyright (c) 2009 Society of Chemical Industry.

Chemical References

• Antineoplastic Agents, Phytogenic
• Carbohydrates
• Mitogens
• Phytohemagglutinins
• Reverse Transcriptase Inhibitors
• Concanavalin A
• DEAE-Cellulose
• reverse transcriptase, Human immunodeficiency virus 1
• HIV Reverse Transcriptase

Topics

• Adsorption
• Antineoplastic Agents, Phytogenic (chemistry, isolation & purification, pharmacology)
• Carbohydrates
• Carcinoma, Hepatocellular (drug therapy)
• Cell Proliferation (drug effects)
• Chromatography (methods)
• Concanavalin A
• DEAE-Cellulose
• HIV Reverse Transcriptase (antagonists & inhibitors)
• Hep G2 Cells
• Hot Temperature
• Humans
• Hydrogen-Ion Concentration
• Mitogens (chemistry, isolation & purification, pharmacology)
• Molecular Structure
• Phaseolus (chemistry)
• Phytohemagglutinins (chemistry, isolation & purification, pharmacology)
• Reverse Transcriptase Inhibitors (chemistry, isolation & purification, pharmacology)
• Spleen (cytology, drug effects)