Long-term downregulation of protease-activated receptor-2 expression in distal colon in rats following bacillary dysentery.
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| Abstract |
The aim of this study was to determine changes of PAR-2 expression in distal colon and the sensitivity of colonic muscle to SLIGRL-NH2, the PAR-2-activating peptide (PAR-2-AP) following bacillary dysentery. Shigella flexneri was administrated intragastrically in healthy male rats to induce bacillary dysentery. The effect of SLIGRL-NH2 on the motility of colonic muscle strips were examined. The expression of PAR-2 was determined by immunohistochemistry and Western blotting. Intragastric administration of S.flexneri induced acute inflammation at the mucosa of the distal colon at 4-11 days, and the inflammation disappeared 18 days later. PAR-2-AP-induced TTX insensitive relaxation of the colonic muscle strips. This inhibitory effect on colonic circular muscle strips was reduced on days 11-35, but the carbachol-induced contraction did not change. PAR-2 was located at the colon smooth muscles cells and myenteric nerve plexus. The amount of PAR-2 expression in distal colon was down regulated on days 11-35. These data indicated that bacillary dysentery exerted a long-term downregulation on the expression of PAR-2 in distal colon. This might be the reason of the low sensitivity of the colon circular muscle strips to the PAR-2-AP-induced relaxation following intragastric administration of S.flexneri.
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| Authors | Yan Luo, Fang Wang, Junfang Qin, Mei Feng, Yinglian Lv, Qian Wang, Kejing Liu, Chuanyong Liu |
| Journal | Regulatory peptides (Regul Pept) Vol. 163 Issue 1-3 Pg. 49-56 (Aug 09 2010) ISSN: 1873-1686 [Electronic] Netherlands |
| PMID | 20347884 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | Copyright (c) 2010 Elsevier B.V. All rights reserved. |
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