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Uptake of dihomo-gamma-linolenic acid by murine macrophages increases series-1 prostaglandin release following lipopolysaccharide treatment.

Abstract
Administration of dihomo-gamma-linolenic acid is useful for atopic dermatitis and atherosclerosis in mice; however, the metabolites of dihomo-gamma-linolenic acid have been little studied. We employed a method which enabled simultaneous analysis of nine prostaglandins using liquid chromatography-tandem mass spectrometry, and determined the concentrations of prostaglandins in the supernatants of cultures of mouse peritoneal macrophages stimulated with lipopolysaccharide after pre-incubation with dihomo-gamma-linolenic acid, arachidonic acid, or eicosapentaenoic acid. Accumulated prostaglandin concentrations from mouse macrophages with dihomo-gamma-linolenic acid uptake increased in a dihomo-gamma-linolenic acid concentration-dependent fashion. These increases were mainly due to prostaglandin D(1) and prostaglandin E(1). The order of accumulated prostaglandin concentrations was dihomo-gamma-linolenic acid>arachidonic acid>eicosapentaenoic acid in supernatants with the same concentration of polyunsaturated fatty acid. Since mouse macrophages can clearly produce series-1 prostaglandins, they must be formed in vivo. These findings suggest that the effects of dihomo-gamma-linolenic acid on diseases may be due to series-1 prostaglandins.
AuthorsSaki Kakutani, Hiroshi Kawashima, Takao Tanaka, Akiko Shiraishi-Tateishi, Yoshinobu Kiso
JournalProstaglandins, leukotrienes, and essential fatty acids (Prostaglandins Leukot Essent Fatty Acids) Vol. 83 Issue 1 Pg. 23-9 (Jul 2010) ISSN: 1532-2823 [Electronic] Scotland
PMID20347284 (Publication Type: Journal Article)
CopyrightCopyright 2010 Elsevier Ltd. All rights reserved.
Chemical References
  • Eicosanoic Acids
  • Lipopolysaccharides
  • Prostaglandins D
  • Arachidonic Acid
  • Alprostadil
  • 8,11,14-Eicosatrienoic Acid
Topics
  • 8,11,14-Eicosatrienoic Acid (pharmacology)
  • Alprostadil (metabolism)
  • Animals
  • Arachidonic Acid (pharmacology)
  • Dermatitis, Atopic (drug therapy, metabolism)
  • Dose-Response Relationship, Drug
  • Eicosanoic Acids (pharmacology)
  • Humans
  • Lipopolysaccharides (pharmacology)
  • Macrophages, Peritoneal (cytology, metabolism)
  • Mice
  • Mice, Inbred BALB C
  • Prostaglandins D (metabolism)

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