Abstract |
Hypertrehalosemic hormone (a carbohydrate-mobilizing neuroendocrine decapeptide) and starvation markedly increased levels of a cockroach (Blaberus discoidalis) fat body cytochrome P450 message. The gene represented by the cloned P450 cDNA has been named CYP4C1 ( cytochrome P450 family 4, subfamily C, gene 1), a newly identified member of the ubiquitous cytochrome P450 monooxygenase gene superfamily. Blaberus CYP4C1 (511 amino acids, Mr = 58,485) has a hydrophobic NH2 terminus and a sequence near the COOH terminus that is homologous to the cysteine-containing heme-binding region definitive of cytochromes P450. The cockroach sequence is 32-36% identical to mammalian family 4A and 4B enzymes. It contains a 13-residue sequence characteristic of family 4 but not other P450s. This study suggests that CYP4C1 is hormonally regulated in association with energy substrate mobilization and supports the idea that family 4 is an old and widespread gene family.
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Authors | J Y Bradfield, Y H Lee, L L Keeley |
Journal | Proceedings of the National Academy of Sciences of the United States of America
(Proc Natl Acad Sci U S A)
Vol. 88
Issue 10
Pg. 4558-62
(May 15 1991)
ISSN: 0027-8424 [Print] United States |
PMID | 2034694
(Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Insect Hormones
- Neuropeptides
- hypertrehalosemic neuropeptide
- DNA
- Cytochrome P-450 Enzyme System
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Topics |
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- Cockroaches
(genetics)
- Cytochrome P-450 Enzyme System
(genetics)
- DNA
(genetics, isolation & purification)
- Gene Expression Regulation
(drug effects)
- Humans
- Insect Hormones
(pharmacology)
- Male
- Molecular Sequence Data
- Neuropeptides
(pharmacology)
- Nucleic Acid Hybridization
- Rats
- Sequence Homology, Nucleic Acid
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