Substrate specific stimulation of NEIL1 by WRN but not the other human RecQ helicases.

Abstract

NEIL1, the mammalian homolog of Escherichia coli endonuclease VIII, is a DNA glycosylase that repairs ring-fragmented purines, saturated pyrimidines and several oxidative lesions like 5-hydroxyuracil, 5-hydroxycytosine, etc. Previous studies from our laboratory have shown that Werner Syndrome protein (WRN), one of the five human RecQ helicases, stimulates NEIL1 DNA glycosylase activity on oxidative DNA lesions. The goal of this study was to extend this observation and analyze the interaction between NEIL1 and all five human RecQ helicases. The DNA substrate specificity of the interaction between WRN and NEIL1 was also analyzed. The results indicate that WRN is the only human RecQ helicase that stimulates NEIL1 DNA glycosylase activity, and that this stimulation requires a double-stranded DNA substrate.

• Authors: Venkateswarlu Popuri, Deborah L Croteau, Vilhelm A Bohr
• Journal: DNA repair (DNA Repair (Amst)) Vol. 9 Issue 6 Pg. 636-42 (Jun 04 2010) ISSN: 1568-7856 [Electronic] Netherlands
• PMID: 20346739 (Publication Type: Journal Article, Research Support, N.I.H., Intramural)
• Copyright: Copyright 2010. Published by Elsevier B.V.

Chemical References

• Oligodeoxyribonucleotides
• Pyrimidines
• 5-hydroxyuracil
• 4,6-diamino-5-N-formamidopyrimidine
• Uracil
• DNA
• Exodeoxyribonucleases
• DNA Glycosylases
• NEIL1 protein, human
• RecQ Helicases
• WRN protein, human
• Werner Syndrome Helicase
• DNA-(Apurinic or Apyrimidinic Site) Lyase
• NEIL2 protein, human

Topics

• Base Sequence
• DNA (genetics, metabolism)
• DNA Damage
• DNA Glycosylases (metabolism)
• DNA-(Apurinic or Apyrimidinic Site) Lyase (metabolism)
• Exodeoxyribonucleases (metabolism)
• Humans
• Oligodeoxyribonucleotides (genetics, metabolism)
• Oxidative Stress
• Protein Binding
• Pyrimidines (metabolism)
• RecQ Helicases (metabolism)
• Substrate Specificity
• Uracil (analogs & derivatives, metabolism)
• Werner Syndrome Helicase