Puerarin inhibits C-reactive protein expression via suppression of nuclear factor kappaB activation in lipopolysaccharide-induced peripheral blood mononuclear cells of patients with stable angina pectoris.

Abstract

Puerarin (4'-7-dihydroxy-8-beta-D-glucosylisoflavone), the most abundant isoflavone-C-glucoside extracted from the root of the plant Pueraria lobata, has demonstrated anti-inflammatory activity in cellular models of inflammation. In this report, we examined the ability of puerarin to modulate C-reactive protein (CRP) expression and key molecules in the nuclear factor kappa B (NF-kappaB) pathway to determine its molecular target. The protein and mRNA levels of CRP were determined in lipopolysaccharide (LPS)-induced peripheral blood mononuclear cells of patients with unstable angina pectoris. Also, we detected the I-kappaBalpha phosphorylation and the p65NF-kappaB expression in peripheral blood mononuclear cells under our experimental condition. The results indicated that puerarin inhibited the expression of the protein and mRNA levels of CRP in LPS-induced peripheral blood mononuclear cells. Subsequently, we determined that the inhibition of CRP expression was because of a dose-dependent inhibition of phosphorylation and degradation of inhibitor kappaB(I-kappaB), which resulted in a reduction of p65NF-kappaB nuclear translocation. We conclude that puerarin acts as an anti-inflammatory agent by blocking NF-kappaB signalling, and may possibly be developed as a useful agent for the chemoprevention of atherosclerosis.

• Authors: Xiangjun Yang, Wenzhi Hu, Qin Zhang, Yueying Wang, Lie Sun
• Journal: Basic & clinical pharmacology & toxicology (Basic Clin Pharmacol Toxicol) Vol. 107 Issue 2 Pg. 637-42 (Aug 2010) ISSN: 1742-7843 [Electronic] England
• PMID: 20346059 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• I-kappa B Proteins
• Isoflavones
• Lipopolysaccharides
• NF-kappa B
• Plant Extracts
• Transcription Factor RelA
• C-Reactive Protein
• puerarin

Topics

• Aged
• Angina Pectoris (blood, drug therapy)
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• C-Reactive Protein (genetics, metabolism)
• Cell Survival (drug effects)
• Cells, Cultured
• Female
• Gene Expression (drug effects)
• Humans
• I-kappa B Proteins (metabolism)
• Isoflavones (pharmacology)
• Leukocytes, Mononuclear (drug effects, metabolism)
• Lipopolysaccharides (pharmacology)
• Male
• Middle Aged
• NF-kappa B (antagonists & inhibitors, metabolism)
• Phosphorylation
• Plant Extracts (pharmacology)
• Pueraria (chemistry)
• Transcription Factor RelA (metabolism)
• Transcriptional Activation (drug effects)