Rat bone marrow stromal cells and oligonucleotides in pain research.

Abstract

In the last years, significant progress has been made in the medical treatment of pain. However, pathological pains, such us neuropathic pain, remain refractory to the currently available analgesics. Therefore, new therapeutic strategies are being evaluated. We have recently shown that both bone marrow stromal cells (MSCs) and the oligonucleotide IMT504 can prevent the development of mechanical and thermal allodynia when they are administered to rats subjected to a sciatic nerve crush. This chapter summarizes the laboratory techniques used to isolate and culture MSCs, administer both MSCs and IMT504, perform the nerve injury and determine mechanical and thermal sensitivities.

• Authors: María Florencia Coronel, Norma Alejandra Chasseing, Marcelo José Villar
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 617 Pg. 327-36 ( 2010) ISSN: 1940-6029 [Electronic] United States
• PMID: 20336432 (Publication Type: Journal Article)

Chemical References

• Oligonucleotides

Topics

• Animals
• Bone Marrow Cells (cytology, physiology)
• Male
• Nerve Crush
• Oligonucleotides (therapeutic use)
• Pain (drug therapy, physiopathology)
• Pain Measurement (instrumentation, methods)
• Rats
• Rats, Sprague-Dawley
• Sciatic Nerve (injuries)
• Stromal Cells (cytology, physiology)