Anthraquinones danthron and quinizarin exert antiproliferative and antimetastatic activity on murine B16-F10 melanoma cells.

Abstract

The anticancer potential of anthraquinones danthron and quinizarin on highly metastatic B16-F10 melanoma murine cells was investigated. Several parameters related to cell proliferation and differentiation, i.e. cell number, intracellular content of polyamines, transglutaminase (EC 2.3.2.13) activity and melanin synthesis were evaluated. Tumor cell growth was strongly reduced after treatment with danthron and quinizarin, as well as polyamine intracellular levels. Moreover, the induction of differentiation in treated cells was demonstrated by the increase of transglutaminase activity. In vitro analysis of adhesion, wound healing migration and invasion showed a remarkable antimetastatic activity of the anthraquinones. The overall results suggest that danthron and quinizarin possess significant antineoplastic properties, probably exerted through the induction of intracellular transglutaminase activity.

• Authors: Stefania Rossi, Claudio Tabolacci, Alessandro Lentini, Bruno Provenzano, Fabrizio Carlomosti, Simona Frezzotti, Simone Beninati
• Journal: Anticancer research (Anticancer Res) Vol. 30 Issue 2 Pg. 445-9 (Feb 2010) ISSN: 1791-7530 [Electronic] Greece
• PMID: 20332452 (Publication Type: Journal Article)

Chemical References

• Anthraquinones
• Melanins
• Mutagens
• Polyamines
• 1,4-dihydroxyanthraquinone
• Transglutaminases
• danthron

Topics

• Animals
• Anthraquinones (pharmacology)
• Blotting, Western
• Cell Adhesion (drug effects)
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Melanins (metabolism)
• Melanoma, Experimental (drug therapy, pathology, secondary)
• Mice
• Mutagens (pharmacology)
• Neoplasm Invasiveness
• Polyamines (metabolism)
• Transglutaminases (metabolism)