Abstract | OBJECTIVE: To observe the effect of human follicular fluid (HFF) from endometriosis (EM) patients treated with Quyu Jiedu Granule (QJG) on mouse embryonic development. METHODS: Cultured 2-cell mouse embryos were divided into three groups. To the medium of Group A (70 embryos), HFF from endometriosis patients treated with QJG was added; to that of Group B (60 embryos), HFF from endometriosis patients untreated with QJG, and to Group C (59 embryos), HFF from patients with fallopian tube obstruction was added. The percentages of embryos developed to 8-cell stage, morula stage and blastula stage were counted, and the early stage number and rate of high-quality embryo were measured. RESULTS: Among the 70 embryos in Group A, 53 (75.71%) developed to 8-cell stage, 48 (68.57%) to morula stage and 45 (64.28%) to blastula stage; while in the 60 embryos of Group B, the corresponding number (percentage) were 34 (56.67%), 29 (48.33%), 21 (35.00%), respectively, showing significant differences between groups (P < 0.05). High-quality of embryo was 61 (87.14%) in Group A and 44 (73.3%) in Group B, the difference between groups also showed statistical significance (P < 0.05). CONCLUSION: HFF from endometriosis patients is toxic to 2-cell mouse embryos, but after treated by QJG, it could elevate the quality of cultured mouse embryo in the early stage.
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Authors | Xin-ling Li, Fang Lian, Yan-he Liu |
Journal | Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine
(Zhongguo Zhong Xi Yi Jie He Za Zhi)
Vol. 29
Issue 11
Pg. 1001-4
(Nov 2009)
ISSN: 1003-5370 [Print] China |
PMID | 20329611
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Drugs, Chinese Herbal
- quyu jiedu
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Topics |
- Animals
- Blastocyst
- Drugs, Chinese Herbal
(therapeutic use)
- Embryo, Mammalian
(drug effects)
- Embryonic Development
(drug effects)
- Endometriosis
(drug therapy)
- Female
- Follicular Fluid
- Humans
- Mice
- Mice, Inbred Strains
- Phytotherapy
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