Abstract |
A lectin with strong mitogenic activity towards human peripheral blood mononuclear cells (PBMCs) and cytotoxic effect on human ovarian cancer cells has been purified from the mycelium of a phytopathogenic fungus, Rhizoctonia bataticola, using ion exchange chromatography and affinity chromatography on asialofetuin- Sepharose. The lectin, termed RBL, is a tetramer of 11-kDa subunits and has unique amino acid sequence at its blocked N-terminus. The purified RBL was blood group nonspecific and its hemagglutination activity was inhibited by mucin (porcine stomach), fetuin ( fetal calf serum) and asialofetuin. Glycan array analysis revealed high affinity binding of RBL towards N- glycans and also the glycoproteins containing complex N- glycan chains. Interestingly, the lectin showed high affinity for glycans which are part of ovarian cancer marker CA125, a high molecular weight mucin containing high mannose and complex bisecting type N-linked glycans as well core 1 and 2 type O- glycans. RBL bound to human PBMCs eliciting strong mitogenic response, which could be blocked by mucin, fetuin and asialofetuin demonstrating the carbohydrate-mediated interaction with the cells. Analysis of the kinetics of binding of RBL to PBMCs revealed a delayed mitogenic response indicating a different signaling pathway compared to phytohemagglutinin-L. RBL had a significant cytotoxic effect on human ovarian cancer cell line, PA-1.
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Authors | Nagaraja N Nagre, Vishwanath B Chachadi, Palaniswamy M Sundaram, Ramachandra S Naik, Radha Pujari, Padma Shastry, Bale M Swamy, Shashikala R Inamdar |
Journal | Glycoconjugate journal
(Glycoconj J)
Vol. 27
Issue 3
Pg. 375-86
(Apr 2010)
ISSN: 1573-4986 [Electronic] United States |
PMID | 20306342
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Glycoproteins
- Lectins
- Polysaccharides
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Topics |
- Animals
- Cell Line
- Cell Line, Tumor
- Cell Survival
(drug effects)
- Chromatography, Gel
- Electrophoresis, Polyacrylamide Gel
- Female
- Flow Cytometry
- Fungi
(chemistry)
- Glycoproteins
(metabolism)
- Hemagglutination Tests
- Humans
- Hydrogen-Ion Concentration
- Lectins
(chemistry, isolation & purification, metabolism, pharmacology)
- Molecular Weight
- Mycelium
(chemistry)
- Ovarian Neoplasms
(metabolism)
- Polysaccharides
(metabolism)
- Protein Binding
- Rabbits
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