The signal peptide of the IgE receptor alpha-chain prevents surface expression of an immunoreceptor tyrosine-based activation motif-free receptor pool.

Abstract

The high affinity receptor for IgE, Fc epsilon receptor I (FcepsilonRI), is an activating immune receptor and key regulator of allergy. Antigen-mediated cross-linking of IgE-loaded FcepsilonRI alpha-chains induces cell activation via immunoreceptor tyrosine-based activation motifs in associated signaling subunits, such as FcepsilonRI gamma-chains. Here we show that the human FcepsilonRI alpha-chain can efficiently reach the cell surface by itself as an IgE-binding receptor in the absence of associated signaling subunits when the endogenous signal peptide is swapped for that of murine major histocompatibility complex class-I H2-K(b). This single-chain isoform of FcepsilonRI exited the endoplasmic reticulum (ER), trafficked to the Golgi and, subsequently, trafficked to the cell surface. Mutational analysis showed that the signal peptide regulates surface expression in concert with other described ER retention signals of FcepsilonRI-alpha. Once the FcepsilonRI alpha-chain reached the cell surface by itself, it formed a ligand-binding receptor that stabilized upon IgE contact. Independently of the FcepsilonRI gamma-chain, this single-chain FcepsilonRI was internalized after receptor cross-linking and trafficked into a LAMP-1-positive lysosomal compartment like multimeric FcepsilonRI. These data suggest that the single-chain isoform is capable of shuttling IgE-antigen complexes into antigen loading compartments, which plays an important physiologic role in the initiation of immune responses toward allergens. We propose that, in addition to cytosolic and transmembrane ER retention signals, the FcepsilonRI alpha-chain signal peptide contains a negative regulatory signal that prevents expression of an immunoreceptor tyrosine-based activation motif-free IgE receptor pool, which would fail to induce cell activation.

• Authors: Barbara Platzer, Edda Fiebiger
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 285 Issue 20 Pg. 15314-15323 (May 14 2010) ISSN: 1083-351X [Electronic] United States
• PMID: 20304923 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers
• Protein Sorting Signals
• Receptors, IgE
• Tyrosine

Topics

• Amino Acid Motifs
• Amino Acid Sequence
• Base Sequence
• Cell Line
• Cell Membrane (metabolism)
• DNA Primers
• Endoplasmic Reticulum (metabolism)
• Flow Cytometry
• Golgi Apparatus (metabolism)
• Humans
• Microscopy, Fluorescence
• Molecular Sequence Data
• Protein Sorting Signals
• Protein Transport
• Receptors, IgE (chemistry, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Tyrosine (metabolism)