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[Expression and function of TRAF1 in Hodgkin's lymphoma cells.].

AbstractOBJECTIVE:
To investigate the function of tumor necrosis factor receptor-associated factor 1 (TRAF1) and CD30-TRAF1 signaling in Hodgkin's lymphoma.
METHODS:
Endogenous and CD30 ligand-induced TRAF1 expression at mRNA and protein levels were examined by quantitative RT-PCR and Western blot analyses, respectively. RNA interference was performed to silence the expression of TRAF1 in L428 cells and examine its effect on cell survival. ELISA-based NF-kappaB family transcription factor activity assay was performed to quantify the kappaB DNA-binding activity in nuclear extracts. The expression of JunB was measured by Western blot.
RESULTS:
TRAF1 expression was detected at both mRNA and protein levels in B cell-derived lymphoma cell lines (L428 and KM-H2). CD30 activation via binding to CD30 ligand induced the TRAF1 expression, the relative mRNA expression was increased to 7.26 +/- 0.23 from 3.50 +/- 0.20, the relative protein expression was increased to 4.53 +/- 0.55 from 2.31 +/- 0.35. The apoptosis rate was increased to (27.7 +/- 5.8)% in TRAF1-silenced L428 cells compared to (5.7 +/- 1.2)% in control cells. The p50 and RelA DNA-binding activity were decreased in TRAF1-silenced L428 cells. The expression of JunB upon CD30 ligand stimulation was not changed in TRAF1-silenced L428 cells.
CONCLUSIONS:
TRAF1 is overexpressed in B cell-derived Hodgkin's lymphoma cells, which is regulated by CD30 signaling pathway. TRAF1 is a crucial molecule mediating the activation of the classical NF-kappaB activity, which further facilitates the anti-apoptosis.
AuthorsWen-Juan Wang, Feng Guo, Ai-Ning Sun, Peng Zhou, Liang Ma
JournalZhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi (Zhonghua Xue Ye Xue Za Zhi) Vol. 31 Issue 1 Pg. 29-33 (Jan 2010) ISSN: 0253-2727 [Print] China
PMID20302775 (Publication Type: Journal Article)
Chemical References
  • NF-kappa B
  • TNF Receptor-Associated Factor 1
Topics
  • Apoptosis
  • Cell Line, Tumor
  • Hodgkin Disease
  • Humans
  • NF-kappa B (metabolism)
  • Signal Transduction (genetics)
  • TNF Receptor-Associated Factor 1

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