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[Nifedipine regulated expression of bcl-2 in human gingival epithelial cells in vitro].

AbstractPURPOSE:
To investigate the effect of nifedipine(NIF) on transcription of bcl-2 in human gingival epithelial cells(HGECs) in vitro and to study the pathogenesis of epithelial thickening in drug-induced gingival overgrowth(DGO).
METHODS:
The gingival tissues obtained from periodontal surgeries were digested with enzyme and HGECs were cultured in vitro; HGECs were identified by immunohistochemistry; bcl-2 mRNA levels were quantitated by Real-time PCR 24 hours and 48 hours after cells stimulated by NIF with different concentration (0microg/ml, 1microg/ml,2microg/ml,3microg/ml), in which 0microg/ml NIF as blank control. The data was analyzed by one-way ANOVA using SPSS 11.0 software package.
RESULTS:
HGECs cultured in vitro showed keratin positive signal and vimentin negative signal; the level of bcl-2 mRNA increased with NIF 3microg/ml after 24 hours treatment, which appeared significant increase compared with blank control (P<0.05); after 48 hours treatment the level of bcl-2 mRNA in the groups of 2microg/ml and 3microg/ml showed significant increase compared with blank control (P<0.05).
CONCLUSION:
NIF regulates the level of bcl-2 mRNA.
AuthorsHai-Yan Wen, Rong Shu, Shao-Yun Jiang, Yun-Tao Jiang
JournalShanghai kou qiang yi xue = Shanghai journal of stomatology (Shanghai Kou Qiang Yi Xue) Vol. 19 Issue 1 Pg. 81-5 (Feb 2010) ISSN: 1006-7248 [Print] China
PMID20300700 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • RNA, Messenger
  • Nifedipine
Topics
  • Epithelial Cells
  • Gingiva
  • Humans
  • In Vitro Techniques
  • Nifedipine
  • RNA, Messenger

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