A self-organized 3-diethylaminopropyl-bearing glycol chitosan nanogel for tumor acidic pH targeting: in vitro evaluation.

Abstract

In this study, a novel pH-responsive nanogel composed of glycol chitosan (GCS) grafted with functional 3-diethylaminopropyl (DEAP) groups (denoted as GCS-g-DEAP hereafter) was fabricated. The GCS-g-DEAP was designed to have a self-assembled arrangement consisting of hydrophilic block (GCS) and hydrophobic block (DEAP) at physiological pH. As the pH decreased to tumor extracellular pH (pH(e)), the nanogel was destabilized due to the protonation of DEAP. The pH-responsive property of the nanogel at tumor extracellular pH (pH(e)) was characterized in drug-release kinetic studies. The release of doxorubicin (DOX) from DOX-loaded nanogels was significantly accelerated at lower pH values, which allowed for increased DOX uptake by non-small lung carcinoma A546 cells under a slightly acidic pH condition, as in tumor pH(e).

• Authors: Nam Muk Oh, Kyung Taek Oh, Hye Jung Baik, Bo Reum Lee, A Hyeong Lee, Yu Seok Youn, Eun Seong Lee
• Journal: Colloids and surfaces. B, Biointerfaces (Colloids Surf B Biointerfaces) Vol. 78 Issue 1 Pg. 120-6 (Jun 15 2010) ISSN: 1873-4367 [Electronic] Netherlands
• PMID: 20299192 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2010 Elsevier B.V. All rights reserved.

Chemical References

• Amines
• Nanogels
• Solutions
• glycol-chitosan
• polyethylene glycol polyethyleneimine nanogel
• Polyethylene Glycols
• Doxorubicin
• Polyethyleneimine
• Chitosan

Topics

• Amines (chemistry)
• Cell Line, Tumor
• Chitosan (analogs & derivatives, chemistry)
• Doxorubicin (pharmacology)
• Drug Delivery Systems (methods)
• Humans
• Hydrogen-Ion Concentration (drug effects)
• Microscopy, Electron, Scanning
• Nanogels
• Neoplasms (metabolism)
• Particle Size
• Polyethylene Glycols (chemistry)
• Polyethyleneimine (chemistry)
• Solutions