Abstract | BACKGROUND & AIMS: METHODS: C57BL/6J mice were fed diets with or without ABA (100mg/kg) for 35 days prior to challenge with 2.5% dextran sodium sulfate (DSS). The severity of clinical disease was assessed daily. Colonic mucosal lesions were evaluated by histopathology, and cellular adhesion molecular and inflammatory markers were assayed by real-time quantitative PCR. Flow cytometry was used to quantify leukocyte populations in the blood, spleen, and mesenteric lymph nodes (MLN). The effect of ABA on cytotoxic T-lymphocyte antigen 4 (CTLA-4) expression in splenocytes was also investigated. RESULTS: ABA significantly ameliorated disease activity, colitis and reduced colonic leukocyte infiltration and inflammation. These improvements were associated with downregulation in vascular cell adhesion marker-1 (VCAM-1), E-selectin, and mucosal addressin adhesion marker-1 (MAdCAM-1) expression. ABA also increased CD4(+) and CD8(+) T-lymphocytes in blood and MLN and regulatory T cells in blood. In vitro, ABA increased CTLA-4 expression through a PPAR γ-dependent mechanism. CONCLUSIONS: We conclude that ABA ameliorates gut inflammation by modulating T cell distribution and adhesion molecule expression.
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Authors | Amir J Guri, Raquel Hontecillas, Josep Bassaganya-Riera |
Journal | Clinical nutrition (Edinburgh, Scotland)
(Clin Nutr)
Vol. 29
Issue 6
Pg. 824-31
(Dec 2010)
ISSN: 1532-1983 [Electronic] England |
PMID | 20236740
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved. |
Chemical References |
- Antigens, CD
- CTLA-4 Antigen
- Cell Adhesion Molecules
- Ctla4 protein, mouse
- E-Selectin
- Madcam1 protein, mouse
- Mucoproteins
- PPAR gamma
- Vascular Cell Adhesion Molecule-1
- Abscisic Acid
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Topics |
- Abscisic Acid
(pharmacology)
- Animals
- Antigens, CD
(metabolism)
- CTLA-4 Antigen
- Cell Adhesion
- Cell Adhesion Molecules
(metabolism)
- Disease Models, Animal
- Down-Regulation
- E-Selectin
(metabolism)
- Inflammatory Bowel Diseases
(immunology, prevention & control)
- Intestinal Mucosa
(immunology, metabolism)
- Leukocytes
(metabolism)
- Mice
- Mice, Inbred C57BL
- Mucoproteins
- Obesity
(pathology)
- PPAR gamma
(metabolism)
- Vascular Cell Adhesion Molecule-1
(metabolism)
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