E-selectin targeting to visualize tumors in vivo.

Abstract

Generally angiogenic factors induce the expression of E-selectin in vascular endothelial cells in the tumors. In this study, we employed an anti-E-selectin monoclonal antibody to target tumors in vivo and evaluated an optical imaging reagent to visualize tumor regions. The anti-E-selectin antibody was conjugated on the surface of liposomes, which encapsulated the near-infrared fluorescent substances Cy3 or Cy5.5. The liposomes efficiently recognized human umbilical vein endothelial cells only when E-selectin was induced by angiogenic factors such as TNF-alpha in vitro. Cy5.5 encapsulated into liposomes that were conjugated with an anti-E-selectin antibody successfully visualized Ehrlich ascites tumor cells when transplanted into mice. Thus, E-selectin targeting with liposomes containing a near-infrared fluorescent dye was found effective in visualizing tumors in vivo. This strategy should be extremely useful as a method to identify sentinel lymphatic nodes and angiogenic tumors as well as use for drug delivery to tumor cells.

• Authors: Masahiko Hirai, Yoshie Hiramatsu, Shinki Iwashita, Takayuki Otani, Ling Chen, Yue-guang Li, Masashi Okada, Kazunori Oie, Koich Igarashi, Hideaki Wakita, Masaharu Seno
• Journal: Contrast media & molecular imaging (Contrast Media Mol Imaging) 2010 Mar-Apr Vol. 5 Issue 2 Pg. 70-7 ISSN: 1555-4317 [Electronic] England
• PMID: 20235150 (Publication Type: Journal Article)
• Copyright: 2010 John Wiley & Sons, Ltd.

Chemical References

• Antibodies, Monoclonal
• E-Selectin
• Immunoconjugates
• Liposomes

Topics

• Animals
• Antibodies, Monoclonal
• Carcinoma, Ehrlich Tumor (diagnosis)
• E-Selectin (immunology)
• Endothelial Cells (cytology)
• Endothelium, Vascular
• Humans
• Immunoconjugates
• Liposomes
• Mice
• Neoplasms (diagnosis, pathology)
• Neovascularization, Pathologic (diagnosis)
• Umbilical Veins