Photodynamic therapy-mediated modulation of inflammatory cytokine production by Epstein-Barr virus-infected nasopharyngeal carcinoma cells.

Abstract

Nasopharyngeal carcinoma (NPC) is a malignant disease associated with Epstein-Barr virus (EBV) infection. This study aims to examine the effects of EBV infection on the production of proinflammatory cytokines in NPC cells after the Zn-BC-AM photodynamic therapy (PDT) treatment. Cells were treated with the photosensitiser Zn-BC-AM for 24 h before light irradiation. Quantitative ELISA was used to evaluate the production of cytokines. Under the same experimental condition, HK-1-EBV cells produced a higher basal level of IL-1alpha (1561 pg/ml), IL-1beta (16.6 pg/ml) and IL-8 (422.9 pg/ml) than the HK-1 cells. At the light dose of 0.25-0.5 J/cm(2), Zn-BC-AM PDT-treated HK-1-EBV cells were found to produce a higher level of IL-1alpha and IL-1beta than the HK-1 cells. The production of IL-1beta appeared to be mediated via the IL-1beta-converting enzyme (ICE)-independent pathway. In contrast, the production of angiogenic IL-8 was downregulated in both HK-1 and HK-1-EBV cells after Zn-BC-AM PDT. Our results suggest that Zn-BC-AM PDT might indirectly reduce tumour growth through the modulation of cytokine production.

• Authors: Ho-Kee Koon, Kwok-Wai Lo, Kwok-Nam Leung, Maria Li Lung, Chris Chi-Kwong Chang, Ricky Ngok-Shun Wong, Wing-Nang Leung, Nai-Ki Mak
• Journal: Cellular & molecular immunology (Cell Mol Immunol) Vol. 7 Issue 4 Pg. 323-6 (Jul 2010) ISSN: 2042-0226 [Electronic] China
• PMID: 20228836 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Inflammation Mediators
• Metalloporphyrins
• Photosensitizing Agents
• Zn-BC-AM

Topics

• Cell Death (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cytokines (biosynthesis)
• Epstein-Barr Virus Infections (immunology, virology)
• Herpesvirus 4, Human (drug effects, immunology)
• Humans
• Inflammation Mediators (metabolism)
• Metalloporphyrins (pharmacology, therapeutic use)
• Nasopharyngeal Neoplasms (drug therapy, pathology, virology)
• Photochemotherapy
• Photosensitizing Agents (pharmacology, therapeutic use)